Publication date: Feb 07, 2019
Tuberculosis, especially multidrug resistant cases, remains an enormous public health threat. Mycobacterium tuberculosis metC (Rv3340) an enzyme involved in methionine biosynthesis was identified and characterized for antimicrobial susceptibility. We reported that the overexpression of Rv3340 in Mycobacterium smegmatis (Ms_Rv3340) produces hydrogen sulfide (HS) for its energy in harsh conditions. The produced HS sustained Ms_Rv3340 against streptomycin whereas the chemical inhibition of HS caused streptomycin lethality to Ms_Rv3340. Further analysis showed that cysteine-HO treatment of Ms-Rv3340 initiated DNA damage via Fenton reaction. Ms_Rv3340 downregulated the expression levels of three streptomycin responsive genes. To our knowledge, no study has been previously reported that M. tuberculosis metC (Rv3340) can generates HS modulating resistant to streptomycin which provides a greater perception toward the treatment and control of tuberculosis.
Lambert, N., Ali, M.K., Wang, X., Huang, X., Yang, W., Duan, X., Yan, S., Li, C., Abdalla, A.E., Jeyakkumar, P., and Xie, J. Mycobacterium tuberculosis metC (Rv3340) derived hydrogen sulfide conferring bacteria stress survival. 04743. 2019 J Drug Target.
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