Plasma-activated medium triggers cell death and the presentation of immune activating danger signals in melanoma and pancreatic cancer cells.

Plasma-activated medium triggers cell death and the presentation of immune activating danger signals in melanoma and pancreatic cancer cells.

Publication date: Mar 11, 2019

Over the past decade, cold atmospheric plasmas have shown promising application in cancer therapy. The therapeutic use of plasma-activated media is a topic addressed in an emerging field known as plasma pharmacy. In oncology, plasma-activated media are used to harness the therapeutic effects of oxidant species when they come in contact with cancer cells. Among several factors that contribute to the anticancer effect of plasma-activated liquid media (PALM), HO and NO derivatives likely play a key role in the apoptotic pathway. Despite the significant amount of literature produced in recent years, a full understanding of the mechanisms by which PALM exert their activity against cancer cells is limited. In this paper, a sealed dielectric-barrier discharge was used to disentangle the effect of reactive nitrogen species (RNS) from that of reactive oxygen species (ROS) on cancer cells. Two cancers characterized by poor prognosis have been investigated: metastatic melanoma and pancreatic cancer. Both tumour models exposed to PALM rich in HO showed a reduction in proliferation and an increase in calreticulin exposure and ATP release, suggesting the potential use of activated media as an inducer of immunogenic cell death via activation of the innate immune system.

Open Access PDF

Azzariti, A., Iacobazzi, R.M., Di Fonte, R., Porcelli, L., Gristina, R., Favia, P., Fracassi, F., , Trizio, Silvestris, N., Guida, G., Tommasi, S., and Sardella, E. Plasma-activated medium triggers cell death and the presentation of immune activating danger signals in melanoma and pancreatic cancer cells. 21943. 2019 Sci Rep (9):1.

Concepts Keywords
Apoptotic Dielectric
ATP T cell
Calreticulin Melanoma
Immunogenic Immune system
Inducer Health
Innate Immune System Immunology
Melanoma Medicine
Nitrogen Medical specialties
Oncology Danger signals melanoma
Oxidant Media topic
Oxygen
Pancreatic Cancer
Pharmacy
Plasma
Plasmas
Prognosis
ROS
Sci
Tumour

Semantics

Type Source Name
disease MESH microsatellite instability
gene UNIPROT MAP2K7
drug DRUGBANK Gold
gene UNIPROT COL17A1
drug DRUGBANK Water
pathway BSID Immune System
disease MESH diagnosis
gene UNIPROT MENT
drug DRUGBANK Trestolone
drug DRUGBANK Albendazole
gene UNIPROT MAP3K10
gene UNIPROT MSTO1
gene UNIPROT MPST
disease DOID msT
gene UNIPROT MARK1
gene UNIPROT SSRP1
gene UNIPROT OCA2
gene UNIPROT B3GALNT1
drug DRUGBANK Proline
disease MESH **p
gene UNIPROT PRDM6
gene UNIPROT NOVA2
gene UNIPROT RRAD
gene UNIPROT MAP1LC3A
gene UNIPROT WASHC1
gene UNIPROT MBOAT4
drug DRUGBANK Human Serum Albumin
gene UNIPROT CES2
gene UNIPROT CASP1
gene UNIPROT NOL3
drug DRUGBANK Fosfomycin
gene UNIPROT TSPO
gene UNIPROT CHRM3
gene UNIPROT ENG
gene UNIPROT C2
drug DRUGBANK Streptomycin
drug DRUGBANK L-Glutamine
drug DRUGBANK D-glucose
drug DRUGBANK Dextrose unspecified form
gene UNIPROT WDFY2
disease DOID HBL
gene UNIPROT BRAF
gene UNIPROT DYRK3
gene UNIPROT IK
gene UNIPROT KIT
drug DRUGBANK Hydrogen peroxide
disease MESH varia
drug DRUGBANK Dapsone
gene UNIPROT FBN1
gene UNIPROT MKKS
gene UNIPROT MKS1
gene UNIPROT RIMS1
gene UNIPROT RBBP8
gene UNIPROT RASA1
gene UNIPROT RGS6
gene UNIPROT DEPP1
gene UNIPROT GOPC
gene UNIPROT LIF
gene UNIPROT DIAPH2
drug DRUGBANK Copper
gene UNIPROT GRAP2
drug DRUGBANK Aluminium
drug DRUGBANK Nitrite
pathway BSID Angiogenesis
disease MESH pathological processes
drug DRUGBANK Nitric Oxide
gene UNIPROT SLC35G1
disease DOID dish
gene UNIPROT THOP1
gene UNIPROT COL9A3
gene UNIPROT COMP
gene UNIPROT COL9A1
gene UNIPROT COL9A2
gene UNIPROT SCN8A
gene UNIPROT F12
gene UNIPROT SLC6A8
gene UNIPROT CALCR
gene UNIPROT CALR
gene UNIPROT GNPTAB
gene UNIPROT FBXO8
gene UNIPROT FBRS
drug DRUGBANK Cisplatin
drug DRUGBANK Paclitaxel
gene UNIPROT CD44
gene UNIPROT MTOR
gene UNIPROT PIK3CG
gene UNIPROT PIK3CB
gene UNIPROT PIK3CD
gene UNIPROT PIK3CA
pathway BSID Signal Transduction
gene UNIPROT AKT1
disease MESH glioblastoma
gene UNIPROT APOPT1
drug DRUGBANK Spinosad
disease MESH recurrence
disease MESH complications
gene UNIPROT FOXD3
disease MESH separated
drug DRUGBANK Medical air
gene UNIPROT MAGEE1
gene UNIPROT TNFSF14
pathway BSID Apoptosis
disease MESH multiple
gene UNIPROT FBXL15
gene UNIPROT FBXW7
gene UNIPROT CNR1
gene UNIPROT SLC26A5
gene UNIPROT SERPINB6
gene UNIPROT SORBS1
gene UNIPROT BRD4
gene UNIPROT HACD1
gene UNIPROT LNPEP
gene UNIPROT CAP1
drug DRUGBANK Aspartame
gene UNIPROT DUOXA1
gene UNIPROT GAST
gene UNIPROT PAGR1
gene UNIPROT GALNS
drug DRUGBANK Oxygen
drug DRUGBANK Nitrogen
disease MESH death
drug DRUGBANK Coenzyme M
pathway BSID Innate Immune System
pathway BSID Release
gene UNIPROT ATP8A2
drug DRUGBANK ATP
gene UNIPROT ROS1
drug DRUGBANK Rosoxacin
gene UNIPROT FAM20C
gene UNIPROT PALM
disease DOID cancer
disease MESH cancer
gene UNIPROT EHD1
pathway BSID Pancreatic cancer
disease DOID pancreatic cancer
disease MESH pancreatic cancer
pathway BSID Melanoma
disease DOID melanoma
disease MESH melanoma
gene UNIPROT ITPRIP

Original Article

Leave a Comment

Your email address will not be published. Required fields are marked *