Towards in vivo melanin radicals detection in melanomas by electron paramagnetic resonance (EPR) spectroscopy: a proof-of-concept study.

Towards in vivo melanin radicals detection in melanomas by electron paramagnetic resonance (EPR) spectroscopy: a proof-of-concept study.

Publication date: Mar 13, 2019

Melanoma is the most aggressive skin tumor type. Although complete cure can be achieved when the whole tumor is resected, prognostic dramatically drops when melanoma cells reach deeper tissues and lymph nodes. Hence, there is an urgent need to develop accurate tools allowing (i) discriminating benign nevi from malignant tumors and (ii) being able to characterize melanoma infiltration. For that purpose, we exploited the paramagnetic properties of melanin by using electron paramagnetic resonance (EPR) spectroscopy to measure the melanin content in pigmented (B16F10 cancer cells) and nonpigmented melanomas (WM2664 cancer cells) inoculated intradermally in nude mice. Specifically, we took advantage of a new clinical EPR device (1 GHz), which provides sensitive measurements of radical species in vivo. Results showed that the melanin-specific EPR signal increased with tumor growth in pigmented tumors, whereas no EPR signal could be detected in achromic melanomas. These data plead for the development of new EPR spectrometers/imagers with improved in-depth resolution for the detection of invasive melanomas.

Desmet, C.M., Danhier, P., Acciardo, S., Lev^eque, P., and Gallez, B. Towards in vivo melanin radicals detection in melanomas by electron paramagnetic resonance (EPR) spectroscopy: a proof-of-concept study. 21955. 2019 Free Radic Res.

Concepts Keywords
Benign Spectroscopy
Electron Paramagnetic Resonance Melanin
EPR EPR
EPR Spectroscopy Nevus
Lymph Nodes Electron paramagnetic resonance
Malignant RTT
Melanin Cancer
Melanoma Spectroscopy
Melanomas Dermatology
Nevi Skin
Paramagnetic Organ systems
Tumor Melanoma
Achromic melanomas

Semantics

Type Source Name
gene UNIPROT ESR1
disease MESH growth
gene UNIPROT NDE1
disease DOID cancer
disease MESH nevi
disease MESH tumor
pathway BSID Melanoma
disease DOID Melanoma
gene UNIPROT EREG
disease MESH melanomas

Original Article

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