Clinical impact of a cytological screening system using cyclin D1 immunostaining and genomic analysis for the diagnosis of thyroid nodules.

Clinical impact of a cytological screening system using cyclin D1 immunostaining and genomic analysis for the diagnosis of thyroid nodules.

Publication date: Mar 18, 2019

Fine-needle aspiration (FNA) is the most reliable method for diagnosing thyroid nodules; however, some features such as atypia of undetermined significance or follicular lesion of undetermined significance can confound efforts to identify malignancies. Similar to BRAF, cyclin D1 may be a strong marker of cell proliferation.

One hundred two patients with thyroidal nodule were enrolled in this prospective study. Expression of cyclin D1 in thyroid nodules was determined by immunohistochemistry using both surgical specimens and their cytological specimens. The identification of the optimal cut off points for the diagnosis of malignancy were evaluated using the receiver operating characteristic (ROC) curves and the assessment of the area under the ROC curve (AUC). The specificity, sensitivity, positive predictive value (PPV) of markers were evaluated from crosstabs based on cut off points and significance were calculated. We also analyzed genetic variants by target NGS for thyroid nodule samples.

The positive predictive value (PPV) and median stain ratio (MSR) of cyclin D1 nuclear staining was determined in papillary thyroid carcinoma (PPV = 91.5%, MSR = 48.5%), follicular adenoma (PPV = 66.7%, MSR = 13.1%), and adenomatous goiter and inflammation controls (MSR = 3.4%). In FNA samples, a threshold of 46% of immunolabelled cells allows to discriminate malignant lesions from benign ones (P 

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Teshima, M., Tokita, K., Ryo, E., Matsumoto, F., Kondo, M., Ikegami, Y., Shinomiya, H., Otsuki, N., Hiraoka, N., Nibu, K.I., Yoshimoto, S., and Mori, T. Clinical impact of a cytological screening system using cyclin D1 immunostaining and genomic analysis for the diagnosis of thyroid nodules. 04258. 2019 BMC Cancer (19):1.

Concepts Keywords
Adenomatous Goitre
AUC Papillary thyroid cancer
Benign Thyroid neoplasm
BMC Endocrine diseases
BRAF Thyroid nodule
Cyclin Anatomical pathology
Cytological Endocrine system
Fine Needle Aspiration Anatomy
FNA Glands
Follicular Thyroid disease
Follicular Adenoma NGS thyroid nodule
Genetic Adenomatous goiter inflammation
Goiter
Immunohistochemistry
Immunostaining
Inflammation
Lesion
Malignancies
Malignancy
Malignant
Nodule
Nodules
Papillary Thyroid Carcinoma
Receiver Operating Characteristic
ROC
ROC Curve
Staining
Thyroid
Thyroid Nodule

Semantics

Type Source Name
disease DOID thyroid carcinoma
gene UNIPROT DEPP1
gene UNIPROT GOPC
gene UNIPROT CYCS
gene UNIPROT MENT
drug DRUGBANK Trestolone
gene UNIPROT PSMB8
gene UNIPROT ARNTL
gene UNIPROT PSD4
disease MESH tic
gene UNIPROT SH3PXD2A
gene UNIPROT SYNM
gene UNIPROT FYN
gene UNIPROT RIOX2
gene UNIPROT NAA50
gene UNIPROT PHF5A
gene UNIPROT MIXL1
gene UNIPROT GEN1
gene UNIPROT AKT1
gene UNIPROT CTNNB1
gene UNIPROT HRAS
gene UNIPROT TP53
gene UNIPROT PTPN5
gene UNIPROT TSPO
gene UNIPROT CHRM3
gene UNIPROT KIT
gene UNIPROT TRIM13
gene UNIPROT CXADR
gene UNIPROT CASR
gene UNIPROT NR1I3
gene UNIPROT PRKAR1A
gene UNIPROT SPG7
disease DOID carcinoma
disease DOID neoplasm
gene UNIPROT POC1A
gene UNIPROT NDP
gene UNIPROT PIEZO1
gene UNIPROT MIB1
gene UNIPROT SP4
disease DOID laryngeal carcinoma
gene UNIPROT ATM
drug DRUGBANK Timonacic
gene UNIPROT MT1A
gene UNIPROT NTRK1
disease DOID medullary thyroid carcinoma
gene UNIPROT PNKD
gene UNIPROT PDC
disease MESH carcinomas
disease MESH papillary carcinomas
gene UNIPROT ABCC8
drug DRUGBANK Formaldehyde
gene UNIPROT AKAP13
disease DOID malignant tumor
gene UNIPROT FBXW11
gene UNIPROT TBX5
gene UNIPROT DNMT1
gene UNIPROT CD69
gene UNIPROT CD5L
disease DOID thyroid neoplasm
gene UNIPROT CCDC6
gene UNIPROT TAS2R38
gene UNIPROT PTCH1
gene UNIPROT RET
gene UNIPROT F9
disease DOID malignant melanoma
disease MESH malignant melanoma
pathway BSID S Phase
gene UNIPROT THEG
gene UNIPROT CCND1
disease DOID adenoma
pathway BSID Aging
disease MESH aging
disease MESH thyroid disease
gene UNIPROT SLC12A3
gene UNIPROT LITAF
disease MESH metastasis
disease MESH thyroid neoplasms
gene UNIPROT PCNT
disease MESH inflammation
disease DOID goiter
disease MESH goiter
disease DOID follicular adenoma
disease MESH follicular adenoma
disease DOID papillary thyroid carcinoma
disease MESH papillary thyroid carcinoma
gene UNIPROT MTRR
gene UNIPROT PAQR7
drug DRUGBANK Saquinavir
gene UNIPROT BRAF
disease MESH malignancies
disease MESH thyroid nodules
disease MESH diagnosis
gene UNIPROT IMPACT

Original Article

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