Altered expression patterns of complement factor H and miR-146a genes in acute-chronic phases in experimental autoimmune encephalomyelitis mouse.

Altered expression patterns of complement factor H and miR-146a genes in acute-chronic phases in experimental autoimmune encephalomyelitis mouse.

Publication date: Apr 10, 2019

Considerable advances have been made in identification of the involvement of immune modulators in diseases. There is growing evidence on the role of complement pathway in pathogenesis and course of multiple sclerosis (MS). Moreover, it has been recognized that microRNAs (miRNAs) play an essential role in modulation and development of immune response in the central nervous system. We aimed to investigate the expression profile of complement factor H (CFH) and miR-146a genes in experimental autoimmune encephalomyelitis (EAE) mouse model of MS to detect the possible roles of CFH and miR-146a as biomarkers of MS disease stats. Expression of CFH and miR-146a genes in liver and brain tissues of EAE mice was measured in acute and chronic phases of disease compared to matched controls using real-time polymerase chain reaction. In the liver, increased expression of CFH gene was observed in the chronic phase compared to the acute phase. However, no significant difference was observed between acute and chronic phase mice with normal mice, while miR-146a expression was significantly decreased in livers of EAE mice in chronic group compared to acute and control groups. The expression of CFH gene in brain had a significant decrease in acute and chronic phases compared to healthy mice. Taken together, these observations indicate probable implication of complement system and miR-146a in course of immune-related diseases and reveal more facts about the pathogenesis of MS. However, further work is needed to determine protein levels of CFH and other possible targets of miR-146a in serum and cerebrospinal fluid of MS patients.

Gharibi, S., Moghimi, B., Haghmorad, D., Mahmoudi, M.B., Shahvazian, E., Yadegari, M., Yazd, E.F., and Tahoori, M.T. Altered expression patterns of complement factor H and miR-146a genes in acute-chronic phases in experimental autoimmune encephalomyelitis mouse. 17736. 2019 J Cell Physiol.

Concepts Keywords
Biomarkers MicroRNA
Brain Multiple sclerosis
Central Nervous System Factor H
Cerebrospinal Fluid Myelin oligodendrocyte glycoprotein
Complement Gene expression
Complement System Experimental autoimmune encephalomyelitis
Liver Animal testing
Mice Organ systems
MicroRNAs Branches of biology
Modulation MS
Mouse Experimental autoimmune encephalomyelitis
Multiple Sclerosis EAE chronic group
Pathogenesis
Polymerase Chain Reaction
Serum

Semantics

Type Source Name
disease DOID relapsing-remitting multiple sclerosis
disease MESH relapsing-remitting multiple sclerosis
gene UNIPROT CFH
disease MESH CFH
disease MESH development
disease DOID multiple sclerosis
disease MESH multiple sclerosis
disease MESH experimental autoimmune encephalomyelitis
gene UNIPROT MLXIP
gene UNIPROT MYLIP
gene UNIPROT MARCH8

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