Quantitative Dot Blot (QDB) as a universal platform for absolute quantification of tissue biomarkers.

Quantitative Dot Blot (QDB) as a universal platform for absolute quantification of tissue biomarkers.

Publication date: Apr 08, 2019

To circumvent the limitations associated with sandwich ELISA for tissue biomarker quantitation, Quantitative Dot Blot method (QDB) was proposed using antibodies clinically validated for immunohistochemistry (IHC), as this method requires only one primary antibody in the analysis. The protein levels of four breast cancer tissue biomarkers, including Estrogen Receptor (ER), Progesterone Receptor (PR), Ki67 and Her2, were absolutely quantitated successfully in 190 frozen breast tissue biopsies, and the results were further verified with provided IHC results. We propose QDB method as an alternative platform to Sandwich ELISA for absolute quantitation of tissue biomarkers with significantly reduced developing effort and time.

Zhang, W., Yu, G., Zhang, Y., Tang, F., Lv, J., Tian, G., Zhang, Y., Liu, J., Mi, J., and Zhang, J. Quantitative Dot Blot (QDB) as a universal platform for absolute quantification of tissue biomarkers. 04402. 2019 Anal Biochem.

Concepts Keywords
Antibodies Antibodies
Biomarker Breast cancer
Biomarkers Dot blot
Breast HER2/neu
ELISA Immunohistochemistry
ER Biomarkers
Estrogen Clinical medicine
Immunohistochemistry Medicine
Primary Antibody Branches of biology
Progesterone
Sandwich
Tissue Biopsies
Universal Absolute

Semantics

Type Source Name
gene UNIPROT ERBB2
drug DRUGBANK Progesterone
disease MESH Estrogen
pathway BSID Breast cancer
disease DOID breast cancer
disease MESH breast cancer

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