Tumor-associated factors are enriched in lymphatic exudate compared to plasma in metastatic melanoma patients.

Tumor-associated factors are enriched in lymphatic exudate compared to plasma in metastatic melanoma patients.

Publication date: Apr 11, 2019

Liquid biopsies allow monitoring of cancer progression and detection of relapse, but reliable biomarkers in melanoma are lacking. Because secreted factors preferentially drain to lymphatic vessels before dilution in the blood, we hypothesized that lymph should be vastly enriched in cancer biomarkers. We characterized postoperative lymphatic exudate and plasma of metastatic melanoma patients after lymphadenectomy and found a dramatic enrichment in lymphatic exudate of tumor-derived factors and especially extracellular vesicles containing melanoma-associated proteins and miRNAs, with unique protein signatures reflecting early versus advanced metastatic spread. Furthermore, lymphatic exudate was enriched in memory T cells, including tumor-reactive CD137 and stem cell-like types. In mice, lymph vessels were the major route of extracellular vesicle transport from tumors to the systemic circulation. We suggest that lymphatic exudate provides a rich source of tumor-derived factors for enabling the discovery of novel biomarkers that may reflect disease stage and therapeutic response.

Broggi, M.A.S., Maillat, L., Clement, C.C., Bordry, N., Corth’esy, P., Auger, A., Matter, M., Hamelin, R., Potin, L., Demurtas, D., Romano, E., Harari, A., Speiser, D.E., Santambrogio, L., and Swartz, M.A. Tumor-associated factors are enriched in lymphatic exudate compared to plasma in metastatic melanoma patients. 22292. 2019 J Exp Med.

Concepts Keywords
Biomarkers Exudate
Biopsies Metastasis
Blood Cancer pathology
Extracellular Melanoma
Extracellular Vesicles Oncology
Exudate Body fluids
Lymph Lymphatic system
Lymph Vessels Branches of biology
Lymphadenectomy Medical specialties
Lymphatic Vessels Medicine
Melanoma
Memory Cells
Metastatic
Mice
Plasma
Relapse
Stem Cell
Systemic Circulation
Tumor
Vesicle Transport

Semantics

Type Source Name
gene UNIPROT TNFRSF9
disease MESH relapse
disease DOID cancer
pathway BSID Melanoma
disease DOID melanoma
disease MESH melanoma
disease MESH Tumor

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