Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF mutation.

Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF mutation.

Publication date: Apr 11, 2019

Liquid biopsies from cancer patients have the potential to improve diagnosis and prognosis. The assessment of surrogate markers of tumor progression in circulating extracellular vesicles could be a powerful non-invasive approach in this setting. We have characterized extracellular vesicles purified from the lymphatic drainage also known as exudative seroma (ES) of stage III melanoma patients obtained after lymphadenectomy. Proteomic analysis showed that seroma-derived exosomes are enriched in proteins resembling melanoma progression. In addition, we found that the BRAF mutation can be detected in ES-derived extracellular vesicles and its detection correlated with patients at risk of relapse.

Garc’ia-Silva, S., Benito-Mart’in, A., S’anchez-Redondo, S., Hern’andez-Barranco, A., Xim’enez-Emb’un, P., Nogu’es, L., Mazariegos, Brinkmann, K., Amor L’opez, A., Meyer, L., Rodr’iguez, C., Garc’ia-Mart’in, C., Boskovic, J., Let’on, R., Montero, C., Robledo, M., Szumera-Cie’ckiewicz, A., , Kalinowska, Skog, J., Noerholm, M., Mu~noz, J., Ortiz-Romero, P.L., Ruano, Y., Rodr’iguez-Peralto, J.L., Rutkowski, P., and Peinado, H. Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF mutation. 22295. 2019 J Exp Med.

Concepts Keywords
Biopsies Lymphadenectomy
BRAF Extracellular vesicle
Exosomes BRAF
Extracellular Vesicles RTT
Exudative Cell biology
Lymphadenectomy Exosome
Melanoma Vesicles
Mutation Melanoma
Prognosis Cancer
Relapse Medicine
Tumor Branches of biology
III melanoma
Drainage
Surrogate markers melanoma

Semantics

Type Source Name
disease MESH relapse
disease MESH seroma
disease MESH diagnosis
disease DOID cancer
disease MESH cancer
gene UNIPROT BRAF
pathway BSID Melanoma
disease MESH melanoma
disease DOID melanoma

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