Real-Time Measurement of Melanoma Cell-Mediated Human Brain Endothelial Barrier Disruption Using Electric Cell-Substrate Impedance Sensing Technology.

Real-Time Measurement of Melanoma Cell-Mediated Human Brain Endothelial Barrier Disruption Using Electric Cell-Substrate Impedance Sensing Technology.

Publication date: Apr 15, 2019

Electric cell-substrate impedance sensing (ECIS) is an impedance-based method for monitoring changes in cell behaviour in real-time. In this paper, we highlight the importance of ECIS in measuring the kinetics of human melanoma cell invasion across human brain endothelium. ECIS data can be mathematically modelled to assess which component of the endothelial paracellular and basolateral barriers is being affected and when. Our results reveal that a range of human melanoma cells can mediate disruption of human brain endothelium, primarily involving the paracellular route, as demonstrated by ECIS. The sensitivity of ECIS also reveals that the paracellular barrier weakens within 30-60 min of the melanoma cells being added to the apical face of the endothelial cells. Imaging reveals pronounced localisation of the melanoma cells at the paracellular junctions consistent with paracellular migration. Time-lapse imaging further reveals junctional opening and disruption of the endothelial monolayer by the invasive melanoma cells all within several hours. We suggest that the ability of ECIS to resolve changes to barrier integrity in real time, and to determine the route of migration, provides a powerful tool for future studies investigating the key molecules involved in the invasive process of cancer cells.

Anchan, A., Kalogirou-Baldwin, P., Johnson, R., Kho, D.T., Joseph, W., Hucklesby, J., Finlay, G.J., O’Carroll, S.J., Angel, C.E., and Graham, E.S. Real-Time Measurement of Melanoma Cell-Mediated Human Brain Endothelial Barrier Disruption Using Electric Cell-Substrate Impedance Sensing Technology. 22345. 2019 Biosensors (Basel) (9):2.

Concepts Keywords
Basel Kinetics melanoma
Biosensors Range melanoma
Brain Localisation melanoma
Endothelial Min melanoma
Endothelial Cells Lapse imaging
Endothelium Biosensors
Impedance Endothelium
Kinetics Medicine
Melanoma ECIS
Monolayer Melanoma
Substrate Cell biology
Time Paracellular transport
Branches of biology

Semantics

Type Source Name
disease DOID cancer
disease MESH cancer
gene UNIPROT FANCE
gene UNIPROT ELOVL6
disease DOID face
pathway BSID Melanoma
disease DOID Melanoma
disease MESH Melanoma

Original Article

Leave a Comment

Your email address will not be published. Required fields are marked *