Increased elasticity of melanoma cells after low-LET proton beam due to actin cytoskeleton rearrangements.

Increased elasticity of melanoma cells after low-LET proton beam due to actin cytoskeleton rearrangements.

Publication date: May 07, 2019

Cellular response to non-lethal radiation stress include perturbations in DNA repair, angiogenesis, migration, and adhesion, among others. Low-LET proton beam radiation has been shown to induce somewhat different biological response than photon radiation. For example, we have shown that non-lethal doses of proton beam radiation inhibited migration of cells and that this effect persisted long-term. Here, we have examined cellular elasticity and actin cytoskeleton organization in BLM cutaneous melanoma and Mel270 uveal melanoma cells. Proton beam radiation increased cellular elasticity to a greater extent than X-rays and both types of radiation induced changes in actin cytoskeleton organization. Vimentin level increased in BLM cells after both types of radiation. Our data show that cell elasticity increased substantially after low-LET proton beam and persisted long after radiation. This may have significant consequences for the migratory properties of melanoma cells, as well as for the cell susceptibility to therapy.

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Jasi’nska-Konior, K., Wieche’c, O., Sarna, M., Panek, A., Swako’n, J., Michalik, M., Urba’nska, K., and Elas, M. Increased elasticity of melanoma cells after low-LET proton beam due to actin cytoskeleton rearrangements. 22552. 2019 Sci Rep (9):1.

Concepts Keywords
Actin Actin
Adhesion Uveal melanoma
Angiogenesis Ionizing radiation
Beam Cytoskeleton
Cytoskeleton Radiation
Elasticity Radiation therapy
Melanoma Medical physics
Migratory Melanoma
Perturbations Radioactivity
Photon Physical sciences
Proton Radiobiology
Radiation Branches of biology
Sci Radiation
Uveal Melanoma Mel270 uveal melanoma
Vimentin Elasticity melanoma
X Rays

Semantics

Type Source Name
pathway BSID Reproduction
gene UNIPROT ADCY10
gene UNIPROT CDK1
disease DOID sarcoma
disease MESH sarcoma
gene UNIPROT ROM1
gene UNIPROT RUNX2
gene UNIPROT NDE1
gene UNIPROT ERAL1
gene UNIPROT ESR1
gene UNIPROT AOC2
gene UNIPROT RHO
gene UNIPROT RHOD
gene UNIPROT TRIO
disease MESH fibrosis
gene UNIPROT WWTR1
gene UNIPROT TAZ
disease MESH tumorigenesis
gene UNIPROT USP5
gene UNIPROT APPL1
disease MESH Death
gene UNIPROT TP53
disease MESH hypoxia
pathway BSID Malaria
disease DOID malaria
disease MESH malaria
disease MESH Subj
gene UNIPROT GEN1
disease DOID ovarian carcinoma
gene UNIPROT COL9A3
gene UNIPROT COMP
gene UNIPROT COL9A1
gene UNIPROT COL9A2
gene UNIPROT SCN8A
drug DRUGBANK Human vaccinia virus immune globulin
drug DRUGBANK Troleandomycin
gene UNIPROT BRD2
gene UNIPROT F11R
drug DRUGBANK Bismuth subgallate
gene UNIPROT MBNL1
gene UNIPROT DUOXA1
gene UNIPROT NLRP5
gene UNIPROT EHD1
disease DOID posterior uveal melanoma
gene UNIPROT ACTA1
gene UNIPROT ZBTB8OS
drug DRUGBANK Iodine
disease DOID Ocular Melanoma
gene UNIPROT INTU
gene UNIPROT NOVA2
gene UNIPROT SALL1
drug DRUGBANK Tromethamine
drug DRUGBANK Aspartame
gene UNIPROT KIT
gene UNIPROT BLNK
gene UNIPROT HARS
gene UNIPROT ATN1
disease DOID squamous carcinoma
gene UNIPROT TBATA
disease MESH squamous carcinoma
gene UNIPROT SIM2
disease MESH metastases
disease MESH diagnosis
gene UNIPROT SSRP1
gene UNIPROT LARGE1
disease MESH visual
disease MESH tumor
drug DRUGBANK Coenzyme M
gene UNIPROT VIM
disease DOID uveal melanoma
disease MESH uveal melanoma
disease DOID cutaneous melanoma
gene UNIPROT BLM
drug DRUGBANK Bleomycin
pathway BSID Angiogenesis
pathway BSID DNA Repair
drug DRUGBANK Tricyclazole
pathway BSID Melanoma
disease DOID melanoma
disease MESH melanoma
gene UNIPROT TRIM9
gene UNIPROT KAT5
gene UNIPROT ITFG1
gene UNIPROT TIPRL
gene UNIPROT METTL8
gene UNIPROT POC1A
gene UNIPROT GRAP2
gene UNIPROT ARID3A
gene UNIPROT ARID1B
gene UNIPROT THOP1
gene UNIPROT MBOAT4
gene UNIPROT PTPN5
drug DRUGBANK Human Serum Albumin
drug DRUGBANK Formaldehyde
disease DOID dish
gene UNIPROT PNMA2
gene UNIPROT YBX3
gene UNIPROT SOBP
gene UNIPROT TUBE1
gene UNIPROT ADA2
gene UNIPROT TNIP1
gene UNIPROT WDFY2
drug DRUGBANK Streptomycin
gene UNIPROT SMIM10L2A
disease DOID uveal tumor
gene UNIPROT SMIM10L2B
disease MESH lentigo
gene UNIPROT ICAM1
gene UNIPROT LAMB3
gene UNIPROT TNNC1
gene UNIPROT TNC
gene UNIPROT CTBP1
gene UNIPROT DYRK3
gene UNIPROT IK
gene UNIPROT CTCF
gene UNIPROT MARK1
gene UNIPROT OCA2
drug DRUGBANK Proline
gene UNIPROT B3GALNT1
disease MESH **p
drug DRUGBANK Afelimomab
disease DOID carcinoma
disease MESH carcinoma
gene UNIPROT SLC22A3
gene UNIPROT ITK
gene UNIPROT MYH14
disease MESH arcs
gene UNIPROT ADRB2
gene UNIPROT NR1H4
gene UNIPROT BFAR
gene UNIPROT C2
gene UNIPROT PTPRF
gene UNIPROT ROS1
drug DRUGBANK Rosoxacin
gene UNIPROT GAPDH
gene UNIPROT YAP1
gene UNIPROT GNAQ
pathway BSID Notch signaling
gene UNIPROT RHOA
gene UNIPROT ANK1
gene UNIPROT PTEN
gene UNIPROT AKT1
gene UNIPROT PIK3CG
gene UNIPROT PIK3CB
gene UNIPROT PIK3CA
gene UNIPROT PIK3CD
gene UNIPROT AFM
gene UNIPROT SH3YL1
gene UNIPROT RAB35
disease MESH multiple
gene UNIPROT LRP1
gene UNIPROT SLC35G1
gene UNIPROT DEPP1
gene UNIPROT GOPC
gene UNIPROT MENT
drug DRUGBANK Trestolone
disease MESH growth
drug DRUGBANK Ethionamide
gene UNIPROT MAGEE1
gene UNIPROT TNFSF13
gene UNIPROT ANP32B
gene UNIPROT HGS
gene UNIPROT SRSF5

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