Long noncoding RNA DSCAM-AS1 is associated with poor clinical prognosis and contributes to melanoma development by sponging miR-136.

Long noncoding RNA DSCAM-AS1 is associated with poor clinical prognosis and contributes to melanoma development by sponging miR-136.

Publication date: Apr 01, 2019

Accumulating evidence suggests that long non-coding RNAs (lncRNAs) are playing critical roles in tumorigenesis. The present study aimed to investigate the expression pattern and effects of lncRNA DSCAM-AS1 (DSCAM-AS1) that was a newly discovered lncRNA in melanoma.

Real-time quantitative PCR (polymerase chain reaction) was performed to determine the expression of DSCAM-AS1 in melanoma tissues and cell lines. Kaplan-Meier and Cox regression analyses were utilized to assess the association between the DSCAM-AS1 and overall survival of patients in melanoma patients. The CCK-8 assay, colony formation assay, flow cytometry assays, transwell and wound scratch assays were performed to determine the biological function of DSCAM-AS1 in tumor cells behaviors. Then, DSCAM-AS1-specific miRNA was further confirmed using the dual-luciferase reporter assay and Western blotting.

In this research, we showed that the expression of DSCAM-AS1 was significantly upregulated in melanoma samples and cell lines. Clinical investigation indicated that higher expression of DSCAM-AS1 was associated with ulceration and advanced stage and led to significantly poorer survival time. High DSCAM-AS1 expression in melanoma was confirmed to be an independent predictor of poor survival of patients using univariate and multivariate analysis. Functional investigations revealed that knockdown of DSCAM-AS1 inhibited the ability of cell proliferation, colony formation, migration, invasion, whereas promoted cell apoptosis. Furthermore, mechanistic investigations indicated that DSCAM-AS1 could interact with miR-136 and negatively influence the expression of miR-136.

Our findings showed that DSCAM-AS1 is a novel tumor-related molecule involved in melanoma progression as well as a potential prognostic biomarker and therapeutic target.

Huang, Y.L., Xu, Q., and Wang, X. Long noncoding RNA DSCAM-AS1 is associated with poor clinical prognosis and contributes to melanoma development by sponging miR-136. 22553. 2019 Eur Rev Med Pharmacol Sci (23):7.

Concepts Keywords
Apoptosis Apoptosis
Assay Life sciences
Biomarker Branches of biology
Colony Articles
Cox Regression Long non-coding RNA
DSCAM Proteins
Flow Cytometry Melanoma
Interact DSCAM
LncRNA Non-coding RNA
LncRNAs AS1 tumor
Luciferase
Melanoma
MiRNA
Molecule
Multivariate Analysis
Polymerase Chain Reaction
Prognosis
Quantitative PCR
Sci
Tumor
Tumorigenesis
Ulceration
Univariate
Western Blotting

Semantics

Type Source Name
pathway BSID Apoptosis
gene UNIPROT SMIM10L2A
gene UNIPROT SMIM10L2B
disease MESH tumor
drug DRUGBANK Sincalide
gene UNIPROT COX5A
gene UNIPROT COX8A
gene UNIPROT CPOX
disease MESH tumorigenesis
gene UNIPROT MLXIP
gene UNIPROT MYLIP
gene UNIPROT MARCH8
disease MESH development
pathway BSID Melanoma
disease DOID melanoma
disease MESH melanoma
gene UNIPROT PTGDR
gene UNIPROT DSCAM

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