Simultaneous assessment of cell morphology and adhesion using aluminum nanoslit-based plasmonic biosensing chips.

Simultaneous assessment of cell morphology and adhesion using aluminum nanoslit-based plasmonic biosensing chips.

Publication date: May 10, 2019

A variety of physiological and pathological processes rely on cell adhesion, which is most often tracked by changes in cellular morphology. We previously reported a novel gold nanoslit-based biosensor that is capable of real-time and label-free monitoring of cell morphological changes and cell viability. However, the preparation of gold biosensors is inefficient, complicated and costly. Recently, nanostructure-based aluminum (Al) sensors have been introduced for biosensing applications. The Al-based sensor has a longer decay length and is capable of analyzing large-sized mass such as cells. Here, we developed two types of double-layer Al nanoslit-based plasmonic biosensors, which were nanofabricated and used to evaluate the correlation between metastatic potency and adhesion of lung cancer and melanoma cell lines. Cell adhesion was determined by Fano resonance signals that were induced by binding of the cells to the nanoslit. The peak and dip of the Fano resonance spectrum respectively reflected long- and short-range cellular changes, allowing us to simultaneously detect and distinguish between focal adhesion and cell spreading. Also, the Al nanoslit-based biosensor chips were used to evaluate the inhibitory effects of drugs on cancer cell spreading. We are the first to report the use of double layer Al nanoslit-based biosensors for detection of cell behavior, and such devices may become powerful tools for anti-metastasis drug screening in the future.

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Hou, H.S., Lee, K.L., Wang, C.H., Hsieh, T.H., Sun, J.J., Wei, P.K., and Cheng, J.Y. Simultaneous assessment of cell morphology and adhesion using aluminum nanoslit-based plasmonic biosensing chips. 22590. 2019 Sci Rep (9):1.

Concepts Keywords
Adhesion Plasmonic biosensing chips
Aluminum Biosensor chips
Biosensing Drug screening
Biosensor Biosensors
Biosensors Universe
Chips Nature
Correlation Plasmon
Double Layer Biotechnology
Focal Adhesion Biosensors
Gold Biosensor chips
Lung Plasmonic biosensing chips
Melanoma
Metastasis
Metastatic
Morphological
Morphology
Nanostructure
Peak
Plasmonic
Sci
Sensor
Spectrum

Semantics

Type Source Name
pathway BSID Reproduction
pathway BSID Pancreatic cancer
disease DOID pancreatic cancer
disease MESH pancreatic cancer
gene UNIPROT TP53
gene UNIPROT TRAC
gene UNIPROT NCOR2
disease DOID lung adenocarcinoma
disease MESH lung adenocarcinoma
gene UNIPROT UGT8
gene UNIPROT NDE1
gene UNIPROT EGFR
gene UNIPROT APPL1
gene UNIPROT TNIP1
gene UNIPROT ACSS2
gene UNIPROT ACCS
gene UNIPROT PLA2G15
drug DRUGBANK Trihexyphenidyl
gene UNIPROT ACTA1
gene UNIPROT BCAR1
gene UNIPROT MLXIP
gene UNIPROT MYLIP
gene UNIPROT MARCH8
disease MESH growth
gene UNIPROT PKM
disease DOID carcinoma
disease MESH carcinoma
gene UNIPROT FAM155B
drug DRUGBANK Oxygen
gene UNIPROT C2
drug DRUGBANK Tricyclazole
drug DRUGBANK Silicon
gene UNIPROT SPIN1
gene UNIPROT GTF2I
drug DRUGBANK Indium
gene UNIPROT IKZF4
gene UNIPROT PRSS33
gene UNIPROT SLITRK1
gene UNIPROT MTA2
drug DRUGBANK Phenindione
disease DOID PID
gene UNIPROT RXFP2
gene UNIPROT MAGEE1
gene UNIPROT SNAP25
gene UNIPROT HERPUD1
gene UNIPROT DCLK1
gene UNIPROT ADGRL1
gene UNIPROT SSRP1
gene UNIPROT FASTK
drug DRUGBANK Albendazole
gene UNIPROT PSMD5
gene UNIPROT PSMD4
gene UNIPROT ARID3A
gene UNIPROT ARID1B
drug DRUGBANK Pentaerythritol tetranitrate
gene UNIPROT DYRK3
gene UNIPROT IK
drug DRUGBANK Medical air
drug DRUGBANK Water
gene UNIPROT TUBE1
gene UNIPROT WASF1
gene UNIPROT ADHFE1
gene UNIPROT DEPP1
gene UNIPROT STUB1
gene UNIPROT TNFSF14
gene UNIPROT GOPC
gene UNIPROT FBLIM1
gene UNIPROT SPR
gene UNIPROT TACR1
gene UNIPROT PRDM6
gene UNIPROT TNFSF13
gene UNIPROT ANP32B
gene UNIPROT PTK2
gene UNIPROT SRC
drug DRUGBANK L-Tyrosine
gene UNIPROT MMRN1
disease MESH inflammation
gene UNIPROT SUN1
gene UNIPROT NAA50
drug DRUGBANK Coenzyme M
disease MESH metastasis
disease DOID cancer
disease MESH cancer
pathway BSID Focal Adhesion
pathway BSID Focal adhesion
gene UNIPROT PIAS2
gene UNIPROT GRAMD4
gene UNIPROT NCKIPSD
gene UNIPROT TSC22D3
pathway BSID Melanoma
disease DOID melanoma
disease MESH melanoma
disease DOID lung cancer
disease MESH lung cancer
gene UNIPROT FBN1
gene UNIPROT LARGE1
drug DRUGBANK Gold
disease MESH pathological processes
drug DRUGBANK Aluminium

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