Development of a reliable microRNA based electrochemical genosensor for monitoring of miR-146a, as key regulatory agent of neurodegenerative disease.

Development of a reliable microRNA based electrochemical genosensor for monitoring of miR-146a, as key regulatory agent of neurodegenerative disease.

Publication date: May 10, 2019

A MicroRNA (miR) based electrochemical method for quantification of miR-146a, a known biomarker for neurodegenerative disease, was developed. In this bioassay, the capture microRNA (C-miR) was self-assembled on the gold surface and used for quantification of target microRNA (T-miR) of miR-146a. For this purpose, an optimized concentration of C-miR was immobilized on the surface of gold electrode and used for capture of target analyte (T-miR). All of preparation steps were characterized by electrochemical techniques (cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS)) and atomic force microscopy (AFM). At the optimized conditions, the linear dynamic range, limit of quantification and relative standard deviation of the proposed bioassay were obtained as 10 pM to 1 μM, 10 pM and 1.59%, respectively. The unprocessed human serum sample was used as a real sample and the results fully confirm that the designed microRNA based biosensor is capable for detection of miR-146a as neurodegenerative disease biomarker. The developed method offers a more precise and high sensitive tool to be used in clinical applications for early detection of neurodegenerative disease like Alzheimer’s and Parkinson.

Khalilzadeh, B., Rashidi, M., Soleimanian, A., Tajalli, H., Kanberoglu, G.S., Baradaran, B., and Rashidi. Development of a reliable microRNA based electrochemical genosensor for monitoring of miR-146a, as key regulatory agent of neurodegenerative disease. 20758. 2019 Int J Biol Macromol.

Concepts Keywords
AFM Spectroscopy
Alzheimer Branches of biology
Analyte Biosensor
Atomic Force Microscopy Mir 1
Bioassay Gene expression
Biomarker MicroRNA
Biosensor
Cyclic Voltammetry
Dynamic Range
Electrochemical
Gold
Gold Electrode
Impedance
MicroRNA
Neurodegenerative
Neurodegenerative Disease
Parkinson
Relative Standard Deviation
Serum
Spectroscopy

Semantics

Type Source Name
gene UNIPROT AFM
drug DRUGBANK Gold
disease DOID neurodegenerative disease
disease MESH neurodegenerative disease
gene UNIPROT MLXIP
gene UNIPROT MYLIP
gene UNIPROT MARCH8
disease MESH Development

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