MicroRNA expression profiles of neuron-derived extracellular vesicles in plasma from patients with amyotrophic lateral sclerosis.

MicroRNA expression profiles of neuron-derived extracellular vesicles in plasma from patients with amyotrophic lateral sclerosis.

Publication date: Jun 04, 2019

Circulating microRNAs (miRNAs) in peripheral blood have been extensively investigated as biomarkers for early diagnosis and monitoring of disease progression. However, their cellular origin as well as their link to the pathophysiology, especially neurodegenerative disease, remains largely unknown. In the present study, we isolated neuron-derived extracellular vesicles (EVs) in plasma by immunoaffinity purification and comprehensively analyzed their miRNA expression profiles using microarray. A total of 30 miRNAs were differentially regulated in amyotrophic lateral sclerosis (ALS) plasma relative to healthy control plasma. Gene ontology analysis revealed that biological processes implicated in both up-regulated and down-regulated miRNAs were involved in synaptic vesicle-related pathways. Especially, 4 miRNAs in plasma neuro-derived EVs seemed to be regulated in the similar manner as those in formalin-fixed paraffin-embedded motor cortex samples from ALS patients. The target genes for the 4 miRNAs partly overlapped in STX1B, RAB3B, and UNC13A genes. UNC13A has been reported to be associated with increased odds of sporadic ALS in multiple genome-wide association studies. Our data suggest that miRNAs extracted from neuron-derived EVs in plasma reflect miRNA alterations in the brain as potential biomarkers of ALS.

Katsu, M., Hama, Y., Utsumi, J., Takashina, K., Yasumatsu, H., Mori, F., Wakabayashi, K., Shoji, M., and Sasaki, H. MicroRNA expression profiles of neuron-derived extracellular vesicles in plasma from patients with amyotrophic lateral sclerosis. 18256. 2019 Neurosci Lett.

Concepts Keywords
ALS RNA
Amyotrophic Lateral Sclerosis Gene expression
Biomarkers MicroRNA
Blood Extracellular RNA
Brain Exosome
Extracellular Vesicles DNA Chip
Formalin
Genome
Microarray
MicroRNA
MicroRNAs
MiRNA
Motor Cortex
Neurodegenerative Disease
Neuron
Ontology
Paraffin
Pathophysiology
Plasma
Synaptic Vesicle

Semantics

Type Source Name
disease MESH multiple
gene UNIPROT UNC13A
gene UNIPROT RAB3B
gene UNIPROT STX1B
drug DRUGBANK Formaldehyde
gene UNIPROT SOD1
gene UNIPROT IGFALS
disease DOID neurodegenerative disease
disease MESH neurodegenerative disease
disease MESH disease progression
disease MESH diagnosis
disease DOID amyotrophic lateral sclerosis
disease MESH amyotrophic lateral sclerosis

Original Article

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