Proteomics characterization of extracellular vesicles sorted by flow cytometry reveals a disease-specific molecular cross-talk from cerebrospinal fluid and tears in multiple sclerosis.

Proteomics characterization of extracellular vesicles sorted by flow cytometry reveals a disease-specific molecular cross-talk from cerebrospinal fluid and tears in multiple sclerosis.

Publication date: Jun 03, 2019

Several proteomics studies have been conducted to identify new cerebrospinal fluid (CSF) biomarkers in Multiple Sclerosis (MuS). However, the complexity of CSF and its invasive collection, limits its use. Therefore, the goal of biomarker research in MuS is to identify novel distinctive targets in CSF or in easily accessible biofluids. Tears represent an interesting matrix for this purpose, because (1) tears are related to the central nervous system (CNS) and (2) the CNS contains Extracellular Vesicles (EVs) derived from brain cells. These EVs are emerging new biomarkers associated to several neurological disorders. Here we applied an optimized flow cytometer for the identification and subtyping of EVs from CSF and tears. We found, for the first time, microglia-derived and neural-derived EVs in tears. The flow cytometer was used to sort and purify 10 EVs from untouched CSF and tears of MuS patients and healthy subjects. Purified EVs were analyzed with shotgun proteomics analysis, revealing that EVs from both CSF and tears of MuS patients conveyed similar proteins. Our data demonstrated a specific EVs-mediated molecular cross talk between CSF and tears, which opens the door to new diagnostic perspectives for MuS. Data are available via ProteomeXchange with identifier PXD013794. SIGNIFICANCE: Proteomics characterization of released Extracellular Vesicles (EVs) in CSF and tears of Multiple Sclerosis patients represents a pioneering application that helped in recognizing information about the biologically relevant molecules. We found, for the first time, microglia-derived and neural-derived EVs in tears. Moreover, purified EVs revealed that both CSF and tears of Multiple Sclerosis patients conveyed similar proteins involved in inflammation, angiogenesis and immune response signalling. We think that our data will contribute to enhance knowledge in Multiple Sclerosis mechanisms and help in biomarker discovery. Moreover tears represent one of the most convenient body fluid for biomarker discovery in Multiple Sclerosis, since it is an high informative and easy accessible. The opportunity to export such a platform to a territory monitoring plan opens the door to new diagnostic perspectives for Multiple Sclerosis.

Pieragostino, D., Lanuti, P., , Cicalini, Cufaro, M.C., Ciccocioppo, F., Ronci, M., Simeone, P., Onofrj, M., van der Pol, E., Fontana, A., Marchisio, M., and Del Boccio, P. Proteomics characterization of extracellular vesicles sorted by flow cytometry reveals a disease-specific molecular cross-talk from cerebrospinal fluid and tears in multiple sclerosis. 18263. 2019 J Proteomics.

Concepts Keywords
Angiogenesis Biomarkers neurological disorders
Biofluids Disease
Biomarker Inflammation
Biomarkers Branches of biology
Body Fluid Vesicles
Brain Body fluids
Central Nervous System Neurology
Cerebrospinal Fluid Cell biology
Cross Cells
Extracellular Vesicles Extracellular vesicle
Flow Cytometer Cerebrospinal fluid
Flow Cytometry Glia
Inflammation Proteomics
Matrix
Microglia
Multiple Sclerosis
Neurological Disorders
Proteomics
Subtyping

Semantics

Type Source Name
pathway BSID Angiogenesis
disease MESH inflammation
disease MESH neurological disorders
gene UNIPROT LAMC2
gene UNIPROT CSF2
disease DOID multiple sclerosis
disease MESH multiple sclerosis

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