Publication date: Jun 06, 2019
The protein MIA is known to be expressed in melanoma and to support melanoma progression. Interestingly, previous studies also observed the expression of MIA in nevi. Concentrating on these findings, we revealed that MIA expression is correlated with a senescent state in melanocytes. Induction of replicative or oncogene-induced senescence resulted in increased MIA expression in vitro. Notably, MIA-knockdown in senescent melanocytes reduced the percentage of senescence-associated beta-Gal-positive cells and enhanced proliferation. Using the melanoma mouse model Tg(Grm1), MIA-deficient mice supported the impact of MIA on senescence by showing a significantly earlier tumor onset compared to controls. In melanocytes, MIA-knockdown led to a downregulation of the cell cycle inhibitor p21 in vitro and in vivo. In contrast, after induction of hTERT in human melanoma cells, p21 regulation by MIA was lost. In summary, our data show for the first time that MIA is a regulator of cellular senescence in human and murine melanocytes. This article is protected by copyright. All rights reserved.
Feuerer, L., Lamm, S., , Henz, Kappelmann-Fenzl, M., Haferkamp, S., Meierjohann, S., Hellerbrand, C., Kuphal, S., and Bosserhoff, A.K. Role of MIA (melanoma inhibitory activity) in melanocyte senescence. 22915. 2019 Pigment Cell Melanoma Res.