Publication date: Jun 06, 2019
To investigate the role of tumor cytokines/chemokines in melanoma immune response, we estimated proportions of immune cell subsets in melanoma tumors from TCGA, followed by evaluation of association between cytokine/chemokine expression and these subsets. We then investigated association of immune cell subsets, chemokines, and cytokines with patient survival. Finally, we evaluated immune cell TIL score for correlation with melanoma patient outcome in a separate cohort. There was good agreement between RNA-seq estimation of T-cell subset with pathologist-determined TIL score. Expression levels of cytokines IL-12A, IFNG, IL-10, and chemokines CXCL9 and CXCL10 were positively correlated with PDCD1, CTLA-4, and CD8+ T cell subset, but negatively correlated with tumor purity (Bonferroni-corrected P
Fang, S., Xu, T., Xiong, M., Zhou, X., Wang, Y., Haydu, L.E., Ross, M.I., Gershenwald, J.E., Prieto, V.G., Cormier, J.N., Wargo, J., Sui, D., Wei, Q., Amos, C.I., and Lee, J.E. Role of immune response, inflammation and tumor immune response-related cytokines/chemokines in melanoma progression. 22926. 2019 J Invest Dermatol.