T cells producing GM-CSF and IL-13 are enriched in the cerebrospinal fluid of relapsing MS patients.

T cells producing GM-CSF and IL-13 are enriched in the cerebrospinal fluid of relapsing MS patients.

Publication date: Jun 25, 2019

Multiple sclerosis (MS) is a central nervous system (CNS) autoimmune demyelinating disease. Its pathogenesis involves humoral and cellular immunity, with production of pro- and anti-inflammatory cytokines by T cells.

To analyze the cytokine profile of cerebrospinal fluid (CSF) T cells in patients with relapsing-remitting MS (RRMS) and non-inflammatory controls.

T cell cytokine production was analyzed by flow cytometry in CSF samples collected from 34 untreated RRMS patients and 20 age-matched controls. Immunofluorescence studies were performed in spinal cord MS active lesions.

Percentages of CSF-derived IL-17A, IL-17A/IL-22, and IL-17A/GM-CSF producing T cells were significantly higher in RRMS patients compared to controls. Percentages of T cells producing IFN-γ were lower in RRMS patients compared to controls. Patients in relapse showed higher percentages of CD4 T cells producing IL-13 and GM-CSF compared to patients in remission. We found a positive correlation between percentages of IL-13 T cells and the Expanded Disability Status Scale (EDSS; ρ = 0.5; p 

Ghezzi, L., Cantoni, C., Cignarella, F., Bollman, B., Cross, A.H., Salter, A., Galimberti, D., Cella, M., and Piccio, L. T cells producing GM-CSF and IL-13 are enriched in the cerebrospinal fluid of relapsing MS patients. 18416. 2019 Mult Scler.

Concepts Keywords
Autoimmune Medical specialties
CD4 Medicine
Cellular Immunity Branches of biology
Central Nervous System Immunology
Cerebrospinal Fluid Neurology
Correlation Multiple sclerosis
Cytokine Inflammatory cytokine
Cytokines Cerebrospinal fluid
Demyelinating Disease Multiple sclerosis biomarkers
Flow Cytometry MOR103
Humoral
IL 13
Immunofluorescence
Multiple Sclerosis
Pathogenesis
Relapse
Remission
Spinal Cord

Semantics

Type Source Name
gene UNIPROT CD4
disease MESH relapse
gene UNIPROT IL22
gene UNIPROT IL17A
disease DOID relapsing-remitting MS
gene UNIPROT LAMC2
gene UNIPROT CSF2
disease DOID demyelinating disease
disease MESH demyelinating disease
disease DOID Multiple sclerosis
disease MESH Multiple sclerosis
gene UNIPROT IL13
drug DRUGBANK Sargramostim

Similar

Original Article

Leave a Comment

Your email address will not be published. Required fields are marked *