Loss-of-function mutations in the melanocortin-1-receptor (Mc1r) cause disruption of dorso-ventral countershading in teleost fish.

Loss-of-function mutations in the melanocortin-1-receptor (Mc1r) cause disruption of dorso-ventral countershading in teleost fish.

Publication date: Jun 28, 2019

The Melanocortin 1 receptor (MC1R) is the central melanocortin receptor involved in vertebrate pigmentation. Mutations in this gene cause variations in coat coloration in amniotes. Additionally, in mammals MC1R is the main receptor for agouti signaling protein (ASIP), making it the critical receptor for the establishment of dorsal-ventral countershading. In fish, Mc1r is also involved in pigmentation but it has been almost exclusively studied in relation to melanosome dispersion activity and as a putative genetic factor involved in dark/light adaptation. However, its role as the crucial component for the Asip1-dependent control of dorsal-ventral pigmentation remains unexplored. Using CRISPR/Cas9 we created mc1r homozygous knockout zebrafish and found that loss-of-function of mc1r causes a reduction of countershading and a general paling of the animals. We find ectopic development of melanophores and xanthophores, accompanied by a decrease in iridophore numbers in the ventral region of mc1r mutants. We also reveal subtle differences in the role of mc1r in repressing pigment cell development between the skin and scale niches in ventral regions This article is protected by copyright. All rights reserved.

Cal, L., Suarez-Bregua, P., , Braasch, Irion, U., Kelsh, R., Cerd’a-Reverter, J.M., and Rotllant, J. Loss-of-function mutations in the melanocortin-1-receptor (Mc1r) cause disruption of dorso-ventral countershading in teleost fish. 23158. 2019 Pigment Cell Melanoma Res.

Concepts Keywords
Agouti Amelanism
Amniotes Biological pigment
ASIP Zebrafish
Cas9 Melanocortin receptor
Countershading Countershading
CRISPR Chromatophore
Dispersion Melanocortin 1 receptor
Dorsal Hair color
Ectopic Skin pigmentation
Fish G protein-coupled receptors
Genetic Branches of biology
Homozygous
Iridophore
Knockout
MC1R
Mc1r
Melanocortin 1 Receptor
Melanoma
Melanophores
Melanosome
Mutants
Pigment
Receptor
Teleost
Ventral
Vertebrate
Xanthophores
Zebrafish

Semantics

Type Source Name
gene UNIPROT TNFSF14
gene UNIPROT ASIP
gene UNIPROT PARD3
gene UNIPROT SH3PXD2A
gene UNIPROT MC1R
disease MESH development

Original Article

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