Photodynamic therapy of melanoma by blue-light photoactivation of flavin mononucleotide.

Photodynamic therapy of melanoma by blue-light photoactivation of flavin mononucleotide.

Publication date: Jul 04, 2019

Melanoma is one of the most aggressive and lethal form of cancer. Photodynamic therapy (PDT) is a clinically approved technique for cancer treatment, including non-melanoma skin cancer. However, the most of conventional photosensitizers are of low efficacy against melanoma due to the possible dark toxicity at high drug concentrations, melanin pigmentation, and induction of anti-oxidant defense mechanisms. In the current research we propose non-toxic flavin mononucleotide (FMN), which is a water-soluble form of riboflavin (vitamin B2) as a promising agent for photodynamic therapy of melanoma. We demonstrated selective accumulation of FMN in melanoma cells in vivo and in vitro in comparison with keratinocytes and fibroblasts. Blue light irradiation with dose 5 J/cm of melanoma cells pre-incubated with FMN led to cell death through apoptosis. Thus, the IC values of human melanoma A375, Mel IL, and Mel Z cells were in a range of FMN concentration 10-30 uM that can be achieved in tumor tissue under systemic administration. The efficiency of reactive oxygen species (ROS) generation under FMN blue light irradiation was measured in single melanoma cells by a label-free technique using an electrochemical nanoprobe in a real-time control manner. Melanoma xenograft regression in mice was observed as a result of intravenous injection of FMN followed by blue-light irradiation of tumor site. The inhibition of tumor growth was 85-90% within 50 days after PDT treatment.

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Akasov, R.A., Sholina, N.V., Khochenkov, D.A., Alova, A.V., Gorelkin, P.V., Erofeev, A.S., Generalova, A.N., and Khaydukov, E.V. Photodynamic therapy of melanoma by blue-light photoactivation of flavin mononucleotide. 23287. 2019 Sci Rep (9):1.

Concepts Keywords
Apoptosis Food colorings
Cancer Flavins
Defense Mechanisms Health
Electrochemical Enzymes
Fibroblasts RTT
Flavin Medicine
FMN Photodynamic therapy
Intravenous Injection Single melanoma
Keratinocytes Tumor
Melanin Photodynamic therapy melanoma
Melanoma Photodynamic therapy
Oxidant Cofactors
Oxygen Light therapy
PDT Flavin mononucleotide
Photodynamic Therapy Melanoma
Photosensitizers Skin cancer
Regression Apoptosis
Riboflavin
ROS
Sci
Skin Cancer
Time Control
Toxicity
Tumor
Vitamin B2
Vivo
Xenograft

Semantics

Type Source Name
pathway BSID Reproduction
gene UNIPROT CEP55
drug DRUGBANK Hydrogen peroxide
gene UNIPROT UBXN11
gene UNIPROT PDZK1
disease DOID dish
gene UNIPROT FBXO8
gene UNIPROT FBRS
gene UNIPROT PUM3
gene UNIPROT TSPAN33
gene UNIPROT PCSK1N
drug DRUGBANK Streptomycin
drug DRUGBANK L-Glutamine
disease MESH recurrence
gene UNIPROT MENT
drug DRUGBANK Trestolone
gene UNIPROT DYRK3
gene UNIPROT IK
disease DOID breast adenocarcinoma
gene UNIPROT F10
gene UNIPROT FAM110A
gene UNIPROT MT1B
gene UNIPROT PCID2
gene UNIPROT MTTP
disease MESH noma
disease DOID noma
pathway BSID ABC transporters
gene UNIPROT MMP28
drug DRUGBANK Cisplatin
disease MESH B16 melanoma
drug DRUGBANK ANX-510
gene UNIPROT PTPN5
gene UNIPROT CDSN
gene UNIPROT SRPX
gene UNIPROT PNN
drug DRUGBANK Formaldehyde
gene UNIPROT ROS1
drug DRUGBANK Rosoxacin
gene UNIPROT RAB8A
pathway BSID Apoptosis
gene UNIPROT PLA2G15
drug DRUGBANK Gold
disease DOID breast tumor
disease MESH breast tumor
gene UNIPROT SCN11A
gene UNIPROT COL9A3
gene UNIPROT COL9A1
gene UNIPROT COMP
gene UNIPROT COL9A2
gene UNIPROT SCN8A
drug DRUGBANK Tricyclazole
gene UNIPROT GRB2
drug DRUGBANK N-Acetylmethionine
disease MESH dengue
drug DRUGBANK Flavin adenine dinucleotide
gene UNIPROT ACTA1
drug DRUGBANK Meticillin
disease DOID amelanotic melanoma
gene UNIPROT MBNL1
drug DRUGBANK Potassium Chloride
gene UNIPROT CASP8AP2
gene UNIPROT CES2
gene UNIPROT CASP1
gene UNIPROT TUBE1
drug DRUGBANK Rasagiline
gene UNIPROT C2
gene UNIPROT PRDM6
gene UNIPROT SPEF1
gene UNIPROT PROM1
gene UNIPROT CD44
gene UNIPROT SSRP1
gene UNIPROT ABCG2
drug DRUGBANK ATP
gene UNIPROT ATP8A2
gene UNIPROT DEPP1
gene UNIPROT GOPC
gene UNIPROT FRK
gene UNIPROT ADAM10
gene UNIPROT TNIP1
gene UNIPROT NLRP5
gene UNIPROT APPL1
gene UNIPROT ACSS2
gene UNIPROT ACCS
disease DOID melanoma
pathway BSID Melanoma
disease MESH melanoma
gene UNIPROT PDLIM5
disease MESH amelanotic melanoma
gene UNIPROT FILIP1
gene UNIPROT INTU
disease MESH endoplasmic reticulum stress
gene UNIPROT BRD2
drug DRUGBANK Ferrous sulfate anhydrous
gene UNIPROT NAA50
gene UNIPROT SEC14L2
disease DOID cutaneous melanoma
disease MESH phototoxicity
drug DRUGBANK Albendazole
gene UNIPROT STATH
gene UNIPROT KIT
gene UNIPROT BRAF
gene UNIPROT MAP2K7
drug DRUGBANK Temozolomide
drug DRUGBANK Dacarbazine
drug DRUGBANK Ipilimumab
drug DRUGBANK Nivolumab
drug DRUGBANK Pembrolizumab
gene UNIPROT ATM
gene UNIPROT NR4A3
gene UNIPROT KAT5
gene UNIPROT ITFG1
gene UNIPROT TIPRL
gene UNIPROT METTL8
drug DRUGBANK Platinum
drug DRUGBANK Activated charcoal
disease MESH inflammation
gene UNIPROT MAGEE1
disease DOID epidermoid carcinoma
disease MESH epidermoid carcinoma
drug DRUGBANK Temoporfin
drug DRUGBANK Porfimer sodium
gene UNIPROT IL37
drug DRUGBANK Oxygen
disease MESH metastasis
disease MESH death
drug DRUGBANK Coenzyme M
disease MESH growth
gene UNIPROT SMIM10L2A
gene UNIPROT SMIM10L2B
drug DRUGBANK Dimethyl sulfoxide
gene UNIPROT NR1H4
gene UNIPROT ADRB2
gene UNIPROT TSPO
gene UNIPROT BFAR
gene UNIPROT CHRM3
drug DRUGBANK Riboflavin
gene UNIPROT FMN1
drug DRUGBANK Water
gene UNIPROT TNFSF14
drug DRUGBANK Flavin mononucleotide
disease MESH cancer
disease DOID cancer
disease MESH skin cancer
disease DOID skin cancer
gene UNIPROT RXFP2

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