Transnuclear mice reveal Peyer’s patch iNKT cells that regulate B-cell class switching to IgG1.

Transnuclear mice reveal Peyer’s patch iNKT cells that regulate B-cell class switching to IgG1.

Publication date: Jul 15, 2019

Tissue-resident iNKT cells maintain tissue homeostasis and peripheral surveillance against pathogens; however, studying these cells is challenging due to their low abundance and poor recovery from tissues. We here show that iNKT transnuclear mice, generated by somatic cell nuclear transfer, have increased tissue resident iNKT cells. We examined expression of PLZF, T-bet, and RORγt, as well as cytokine/chemokine profiles, and found that both monoclonal and polyclonal iNKT cells differentiated into functional subsets that faithfully replicated those seen in wild-type mice. We detected iNKT cells from tissues in which they are rare, including adipose, lung, skin-draining lymph nodes, and a previously undescribed population in Peyer’s patches (PP). PP-NKT cells produce the majority of the IL-4 in Peyer’s patches and provide indirect help for B-cell class switching to IgG1 in both transnuclear and wild-type mice. Oral vaccination with α-galactosylceramide shows enhanced fecal IgG1 titers in iNKT cell-sufficient mice. Transcriptional profiling reveals a unique signature of PP-NKT cells, characterized by tissue residency. We thus define PP-NKT as potentially important for surveillance for mucosal pathogens.

Clancy-Thompson, E., Chen, G.Z., LaMarche, N.M., Ali, L.R., Jeong, H.J., Crowley, S.J., Boelaars, K., Brenner, M.B., Lynch, L., and Dougan, S.K. Transnuclear mice reveal Peyer’s patch iNKT cells that regulate B-cell class switching to IgG1. 23379. 2019 EMBO J (38):14.

Concepts Keywords
Adipose Vaccination
B Cell Branches of biology
Chemokine Medical specialties
Class Switching Immunology
Cytokine Immune system
EMBO Glycoproteins
Faithfully Immunoglobulin G
Homeostasis Chemokine
IgG1 Nuclear transfer
Lung
Lymph Nodes
Mice
Monoclonal
NKT
Nuclear Transfer
Residency
Somatic
Surveillance
Vaccination
Wild Type

Semantics

Type Source Name
gene UNIPROT IL4
drug DRUGBANK Binetrakin
gene UNIPROT TBX21
gene UNIPROT ZBTB16

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