Do GNAQ and GNA11 Differentially Affect Inflammation and HLA Expression in Uveal Melanoma?

Do GNAQ and GNA11 Differentially Affect Inflammation and HLA Expression in Uveal Melanoma?

Publication date: Aug 07, 2019

Inflammation, characterized by high numbers of infiltrating leukocytes and a high HLA Class I expression, is associated with a bad prognosis in uveal melanoma (UM). We wondered whether mutations in GNA11 or GNAQ differentially affect inflammation and HLA expression, and thereby progression of the disease. We analyzed data of 59 primarily enucleated UM eyes. The type of GNAQ/11 mutation was analyzed using dPCR; chromosome aberrations were determined by Fluorescence in Situ Hybridization (FISH), karyotyping, and single nucleotide polymorphism (SNP) analysis, and mRNA expression by Illumina PCR. Comparing tumors with a GNAQ mutation with those with a GNA11 mutation yielded no significant differences in histopathological characteristics, infiltrate, or HLA expression. When comparing the Q209L mutations with Q209P mutations in tumors with monosomy of chromosome 3, a higher mitotic count was found in the Q209P/M3 tumors (p = 0.007). The Kaplan-Meier (KM) curves between the patients of the different groups were not significantly different. We conclude that the type (Q209P/Q209L) or location of the mutation (GNA11/GNAQ) do not have a significant effect on the immunological characteristics of the tumors, such as infiltrate and HLA Class I expression. Chromosome 3 status was the main determinant in explaining the difference in infiltrate and HLA expression.

van Weeghel, C., Wierenga, A.P.A., Versluis, M., van Hall, T., van der Velden, P.A., Kroes, W.G.M., Pfeffer, U., Luyten, G.P.M., and Jager, M.J. Do GNAQ and GNA11 Differentially Affect Inflammation and HLA Expression in Uveal Melanoma? 23705. 2019 Cancers (Basel) (11):8.

Concepts Keywords
Basel Hybridization
Chromosome Cancer
Chromosome Aberrations Epidemiology
Determinant Medicine
Enucleated Health
FISH Uveal melanoma
Histopathological Rare diseases
HLA RTT
Illumina Melanoma
Inflammation SNP
Karyotyping Single nucleotide polymorphism
Leukocytes
Melanoma
Mitotic
Monosomy
MRNA
Mutation
PCR
Prognosis
Single Nucleotide Polymorphism
SNP
Uveal Melanoma

Semantics

Type Source Name
disease MESH monosomy
disease MESH tumors
gene UNIPROT SH3PXD2A
disease MESH chromosome aberrations
gene UNIPROT BAD
disease DOID Uveal Melanoma
disease MESH Uveal Melanoma
disease MESH Inflammation
gene UNIPROT GNA11
gene UNIPROT GNAQ

Similar

Original Article

Leave a Comment

Your email address will not be published. Required fields are marked *