Inter-and intra-subject variability of potential plasma and urine metabolite and protein biomarkers in healthy human volunteers.

Inter-and intra-subject variability of potential plasma and urine metabolite and protein biomarkers in healthy human volunteers.

Publication date: Aug 10, 2019

A limited understanding of inter- and intra-subject variability hampers effective biomarker translation from in-vitro/in-vivo studies to clinical trials and clinical decision support. Specifically, variability of biomolecule concentration can play an important role in interpretation, power analysis and sampling time designation. In the present study, a wide range of 749 plasma metabolites, 62 urine biogenic amines, and 1263 plasma proteins were analyzed in 10 healthy male volunteers measured repeatedly during 12 hours under tightly-controlled conditions. Three variability components in relative concentration data are determined using Linear Mixed Models: between (inter-subject), time (intra-subject) and noise (intra-subject). Biomolecules such as CMPF, PDGF C, and cathepsin D with low noise potentially detect changing conditions within a person. If also the between component is low, biomolecules can easier differentiate conditions between persons, for example cathepsin D, CD27 antigen, and prolylglycine. Variability over time does not necessarily inhibit translatability, but requires to choose sampling times carefully. This article is protected by copyright. All rights reserved.

Duisters, K., Ogino, S., Andou, T., Ito, K., Akabane, T., Harms, A., Moerland, M., Hashimoto, Y., Ando, A., Ohtsu, Y., Wada, N., Yukinaga, H., Meulman, J., Kobayashi, H., Kobayashi, N., Suzumura, K., and Hankemeier, T. Inter-and intra-subject variability of potential plasma and urine metabolite and protein biomarkers in healthy human volunteers. 05164. 2019 Clin Pharmacol Ther.

Concepts Keywords
Antigen Cathepsin
Biomarker Chemical pathology
Biomarkers Biotechnology
Biomolecule Biomarkers
Biomolecules Branches of biology
Cathepsin Statistical dispersion
Clinical Trials
Metabolite
Noise
PDGF
Plasma
Urine
Vivo

Semantics

Type Source Name
gene UNIPROT CD27
gene UNIPROT CTSD
pathway BSID Translation

Original Article

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