A new series of acetohydroxamates shows in vitro and in vivo anticancer activity against melanoma.

A new series of acetohydroxamates shows in vitro and in vivo anticancer activity against melanoma.

Publication date: Sep 06, 2019

Cancer treatment is challenging, mainly due to high levels of drug toxicity and the resistance of tumours to chemotherapy. Hydroxamic acid derivatives have recently aroused attention due to their potential to treat malignancies. In the present study, we sought to investigate the anticancer effects of a new series of synthetic acetohydroxamates. The in vitro cytotoxic and antiproliferative effects of 11 synthetic acetohydroxamates were evaluated against the melanoma cell line A375. Apoptosis, cell cycle, and autophagy assays were employed to elucidate the cell death pathways induced by the compounds. The in vivo pharmacokinetic profiles of the most promising compounds were determined in CD-1 mice, while the in vivo antitumour efficacies were evaluated using the A375 melanoma xenograft model in nude mice. MTT assays revealed that all compounds presented concentration-dependent cytotoxicity against the A375 cell line. AKS 61 produced the most favourable antiproliferative activity according to the sulphorhodamine B and clonogenic assays. AKS 61 treatment resulted in decreased mitochondrial membrane potential and increased apoptosis and autophagy in the A375 cell line. However, AKS 61 failed to prevent in vivo tumour growth in a melanoma xenograft, whereas compound AKS 7 was able to inhibit tumour growth when administered orally. These in vivo findings may be explained by a more favourable pharmacokinetic profile presented by AKS 7 when compared to AKS 61. Taken together, these results suggest that acetohydroxamates have potential anticancer effects and will guide future optimisation of these molecules to allow for further non-clinical development.

Segat, G.C., Moreira, C.G., Santos, E.C., Heller, M., Schwanke, R.C., Aksenov, A.V., Aksenov, N.A., Aksenov, D.A., Kornienko, A., Marcon, R., and Calixto, J.B. A new series of acetohydroxamates shows in vitro and in vivo anticancer activity against melanoma. 24044. 2019 Invest New Drugs.

Concepts Keywords
Antiproliferative Melanoma treatment
Antitumour Anticancer activity melanoma
Apoptosis A375 melanoma
Autophagy Tumour growth melanoma
Chemotherapy Chemotherapy
Cytotoxic Branches of biology
Cytotoxicity Autophagy
Drug Toxicity Immunology
Hydroxamic Acid Programmed cell death
Malignancies NOV
Melanoma Apoptosis
Membrane Potential Chemotherapy
Mice
Mitochondrial Membrane
Pharmacokinetic
Tumour
Xenograft

Semantics

Type Source Name
drug DRUGBANK Acetohydroxamic acid
disease MESH growth
gene UNIPROT NDE1
pathway BSID Cell Cycle
pathway BSID Cell cycle
pathway BSID Apoptosis
disease MESH drug toxicity
disease DOID Cancer
disease MESH Cancer
pathway BSID Melanoma
disease DOID melanoma
disease MESH melanoma

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