Tobramycin Promotes Melanogenesis by Upregulating p38 MAPK Protein Phosphorylation in B16F10 Melanoma Cells.

Tobramycin Promotes Melanogenesis by Upregulating p38 MAPK Protein Phosphorylation in B16F10 Melanoma Cells.

Publication date: Sep 05, 2019

Tobramycin is an aminoglycoside-based natural antibiotic derived from Streptomyces tenebrarius, which is primarily used for Gram-negative bacterial infection treatment. Although tobramycin has been utilized in clinical practice for a long time, it has exhibited several side effects, leading to the introduction of more effective antibiotics. Therefore, we conducted our experiments focusing on new possibilities for the clinical use of tobramycin. How tobramycin affects skin melanin formation is unknown. This study used B16F10 melanoma cells to assess the effect of tobramycin on melanin production. After cytotoxicity was assessed by MTT assay, melanin content and tyrosinase activity analyses revealed that tobramycin induces melanin synthesis in B16F10 cells. Next, Western blot analyses were performed to elucidate the mechanism by which tobramycin increases melanin production; phosphorylated p38 protein expression was upregulated. Protein inhibitors have been used to elucidate the mechanism of tobramycin. Kanamycin A and B are structurally similar to tobramycin, and 2-DOS represents the central structure of these antibiotics. The effects of these substances on melanogenesis were evaluated. Kanamycin A reduced melanin production, whereas kanamycin B and 2-DOS had no effect. Overall, our data indicated that tobramycin increases melanin production by promoting p38 protein phosphorylation in B16F10 melanoma cells.

Moon, S.H., Chung, Y.C., and Hyun, C.G. Tobramycin Promotes Melanogenesis by Upregulating p38 MAPK Protein Phosphorylation in B16F10 Melanoma Cells. 24049. 2019 Antibiotics (Basel) (8):3.

Concepts Keywords
Aminoglycoside Kanamycin A
Antibiotic Melanoma
Antibiotics Tyrosinase
Bacterial Infection Streptomyces tenebrarius
Basel Melanin
Cytotoxicity Tobramycin
DOS Protein kinases
Gram Negative RTT
Kanamycin Aminoglycoside antibiotics
Melanin Enzymes
Melanogenesis Antibiotics
Melanoma Negative bacterial infection
P38
P38 MAPK
Phosphorylated
Phosphorylation
Streptomyces
Tobramycin
Tyrosinase
Western Blot

Semantics

Type Source Name
drug DRUGBANK Kanamycin
gene UNIPROT GKN2
disease MESH Gram-negative bacterial infection
pathway BSID Melanoma
disease DOID Melanoma
disease MESH Melanoma
gene UNIPROT RNF19A
gene UNIPROT CRK
gene UNIPROT AHSA1
gene UNIPROT GRAP2
gene UNIPROT POLDIP2
pathway BSID Melanogenesis
drug DRUGBANK Tobramycin

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