Sequence and expression analysis of the cytoplasmic pattern recognition receptor melanoma differentiation-associated gene 5 from the barbel chub Squaliobarbus curriculus.

Sequence and expression analysis of the cytoplasmic pattern recognition receptor melanoma differentiation-associated gene 5 from the barbel chub Squaliobarbus curriculus.

Publication date: Sep 05, 2019

MDA5 is a cytoplasmic viral double-stranded RNA recognition receptor that plays a pivotal role in the aquatic animal innate immune system. To decipher the role of MDA5 of Squaliobarbus curriculus (ScMDA5) in the immune response, full-length cDNA of ScMDA5 was cloned using the RACE technology, mRNA and protein expression levels of ScMDA5 signalling pathway members in response to stimulation were detected and effects of overexpression of ScMDA5 on the immune response were investigated. ScMDA5 comprises 3597 bp and is composed of an open reading frame (2958 nucleotides long) that translates into a putative peptide of 985 amino acid residues. ScMDA5 possesses two N-terminal caspase-recruiting domains, DEAD-like helicases superfamily, helicase superfamily C-terminal and RIG-I_C-RD domains, and differences in these domains among species were mainly observed with respect to their length and location. ScMDA5 was closely clustered with those of Carassius auratus, Ctenopharyngodon idellus and Mylopharyngodon piceus. ScMDA5 transcripts were most abundant in the spleen and the lowest in the liver. Expression levels of ScMDA5 in healthy tissues were significantly correlated with those of ScIRF3, ScIRF7 and ScIFN. Besides, mRNA expression levels of ScIRF3 were significantly correlated with those of ScIRF7 (0.956, P 

Li, Y., Jin, S., Zhao, X., Luo, H., Li, R., Li, D., and Xiao, T. Sequence and expression analysis of the cytoplasmic pattern recognition receptor melanoma differentiation-associated gene 5 from the barbel chub Squaliobarbus curriculus. 24053. 2019 Fish Shellfish Immunol.

Concepts Keywords
Amino Acid MDA5
Aquatic Innate immune system
C Terminal Immune system
Carassius Auratus Intracellular receptors
Caspase Technology mRNA expression
CDNA
Cloned
Ctenopharyngodon
Helicase
Immune Response
Innate Immune System
Liver
Melanoma
MRNA
N Terminal
Nucleotides
Peptide
Receptor
Signalling Pathway
Spleen
Superfamily
Viral

Semantics

Type Source Name
gene UNIPROT CA8
gene UNIPROT MINDY3
gene UNIPROT ANKRD1
gene UNIPROT RPS15
gene UNIPROT RIG
gene UNIPROT DIRAS1
pathway BSID Innate Immune System
gene UNIPROT IFIH1
pathway BSID Melanoma
disease DOID melanoma
disease MESH melanoma

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