A new strategy for accurate targeted diagnosis and treatment of cutaneous malignant melanoma: dual-mode phase-change lipid nanodroplets as ultrasound contrast agents.

A new strategy for accurate targeted diagnosis and treatment of cutaneous malignant melanoma: dual-mode phase-change lipid nanodroplets as ultrasound contrast agents.

Publication date: Mar 03, 2019

Currently, effective detection and treatment of cutaneous malignant melanoma (CMM) still face severe challenges. Ultrasound molecular imaging as a noninvasive and easy-to-operate method is expected to bring improvements for tumor detection.

The aim of this research is to prepare novel phase-change ultrasound contrast agents, Nds-IR780, which can perform not only dual-mode molecule-targeted imaging but also targeted photothermal therapy for CMM.

A double emulsion process was used to prepare the Nds-IR780. Then, the entrapment rate and drug loading of IR-780 iodide in Nds-IR780 were detected by high-performance liquid chromatography. The biocompatibility of Nds-IR780 was evaluated by a CCK-8 assay and the characteristics and stability of that were verified through the particle size analyzer, laser scanning confocal microscopy (LSCM) and transmission electron microscopy (TEM). The abilities of dual-mode molecule-targeted imaging and targeted photothermal therapy for Nds-IR780 were confirmed via the in vitro and in vivo experiments.

Nds-IR780 had good size distribution, polydispersity index, stability and biosafety. The in vitro and in vivo experiments confirmed that Nds-IR780 were capable of targeting CMM cells with high affinity (22.4+/-3.2%) and facilitating dual-mode imaging to detect the primary lesion and sentinel lymph nodes (SLNs) of CMM. Furthermore, the photothermal ablation of CMM mediated by Nds-IR780 was very effective in vivo.

The newly prepared Nds-IR780 were observed to be effective targeted theranostic probe for the precise detection and targeted treatment of CMM.

Open Access PDF

Yang, H., Cai, W., Lv, W., Zhao, P., Shen, Y., Zhang, L., Ma, B., Yuan, L., Duan, Y., and Yao, K. A new strategy for accurate targeted diagnosis and treatment of cutaneous malignant melanoma: dual-mode phase-change lipid nanodroplets as ultrasound contrast agents. 24412. 2019 Int J Nanomedicine (14):

Concepts Keywords
Affinity Ultrasound
Assay Melanoma
Biocompatibility Microscopy
Biosafety Photothermal therapy
CMM Molecular imaging
Contrast Agents Light therapy
Emulsion Medical imaging
Entrapment Health
Iodide Clinical medicine
Lesion Medicine
Lipid Improvements tumor
Malignant Melanoma Cutaneous malignant melanoma
Molecular Imaging Dual mode imaging
Molecule Molecular imaging
Nanomedicine Laser
Particle Size Analyzer Ultrasound
Phase Change
Polydispersity
Sentinel Lymph Nodes
TEM
Theranostic
Transmission Electron Microscopy
Tumor
Ultrasound
Vivo

Semantics

Type Source Name
gene UNIPROT TNMD
drug DRUGBANK Tretamine
drug DRUGBANK Sincalide
drug DRUGBANK Iodide
gene UNIPROT DNMT1
gene UNIPROT CD69
disease MESH tumor
gene UNIPROT FANCE
gene UNIPROT ELOVL6
disease DOID face
disease DOID malignant melanoma
disease MESH malignant melanoma
disease MESH diagnosis

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