Immunohistochemistry for PRAME in the Distinction of Nodal Nevi From Metastatic Melanoma.

Immunohistochemistry for PRAME in the Distinction of Nodal Nevi From Metastatic Melanoma.

Publication date: Oct 16, 2019

The distinction of metastatic melanoma from melanocytic nevi in lymph nodes can on occasion be difficult. As diffuse immunohistochemical (IHC) PRAME (PReferentially expressed Antigen in MElanoma) expression is detected in the majority of primary and metastatic melanomas, but rarely in nevi, we reasoned that PRAME could be a useful adjunct marker for the diagnosis of melanocytes in lymph nodes. In this study, we examined 45 nodal melanocytic deposits comprising 30 nodal nevi and 15 melanoma metastases. The latter were diagnostically not straightforward because they either coexisted with nodal nevi or were present in perinodal fibrous tissue. All nodal nevi (30/30) were negative for PRAME, whereas all melanoma metastases (15/15) were diffusely positive for PRAME IHC. We additionally report the novel use of a PRAME/Melan A dual-label immunostain. Our results show that PRAME IHC may be useful in the assessment of diagnostically challenging nodal melanocytic deposits, such as intraparenchymal nodal nevi, metastases confined to the capsular fibrous tissue, or in the setting of small metastases coexisting with a nodal nevus in the same lymph node.

Lezcano, C., Pulitzer, M., Moy, A.P., Hollmann, T.J., Jungbluth, A.A., and Busam, K.J. Immunohistochemistry for PRAME in the Distinction of Nodal Nevi From Metastatic Melanoma. 24516. 2019 Am J Surg Pathol.

Concepts Keywords
Antigen Medicine
Fibrous Tissue Neoplasms
Immunohistochemical Organ systems
Immunohistochemistry RTT
Immunostain Cancer
Lymph Node Melanoma
Lymph Nodes Oncology
Melanocytes Metastasis
Melanocytic Nevi PRAME
Melanoma Nevus
Melanomas Melanocytic nevus
Metastases
Metastatic
Nevi
Nevus
Nodal

Semantics

Type Source Name
disease MESH metastases
disease MESH diagnosis
disease MESH melanocytic nevi
pathway BSID Melanoma
disease DOID Melanoma
disease MESH Melanoma
disease MESH Nevi
gene UNIPROT NODAL
gene UNIPROT PRAME

Original Article

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