A highly specific and sensitive nanoimmunosensor for the diagnosis of neuromyelitis optica spectrum disorders.

A highly specific and sensitive nanoimmunosensor for the diagnosis of neuromyelitis optica spectrum disorders.

Publication date: Nov 06, 2019

A precise diagnosis for neuromyelitis optica spectrum disorders (NMOSD) is crucial to improve patients’ prognostic, which requires highly specific and sensitive tests. The cell-based assay with a sensitivity of 76% and specificity of 100% is the most recommended test to detect anti-aquaporin-4 antibodies (AQP4-Ab). Here, we tested four AQP4 external loop peptides (AQP4, AQP4, AQP4, and AQP4) with an atomic force microscopy nanoimmunosensor to develop a diagnostic assay. We obtained the highest reactivity with AQP4-nanoimunosensor. This assay was effective in detecting AQP4-Ab in sera of NMOSD patients with 100% specificity (95% CI 63.06-100), determined by the cut-off adhesion force value of 241.3 pN. NMOSD patients were successfully discriminated from a set of healthy volunteers, patients with multiple sclerosis, and AQP4-Ab-negative patients. AQP4 sensitivity was 81.25% (95% CI 56.50-99.43), slightly higher than with the CBA method. The results with the AQP4-nanoimmunosensor indicate that the differences between NMOSD seropositive and seronegative phenotypes are related to disease-specific epitopes. The absence of AQP4-Ab in sera of NMOSD AQP4-Ab-negative patients may be interpreted by assuming the existence of another potential AQP4 peptide sequence or non-AQP4 antigens as the antibody target.

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de Souza Moraes, A., Brum, D.G., Ierich, J.C.M., Higa, A.M., Assis, A.S.J., Miyazaki, C.M., Shimizu, F.M., Peroni, L.A., Machini, M.T., Barreira, A.A., Ferreira, M., Oliveira, O.N., and Leite, F.L. A highly specific and sensitive nanoimmunosensor for the diagnosis of neuromyelitis optica spectrum disorders. 19430. 2019 Sci Rep (9):1.

Concepts Keywords
Ab Negative Disease
Adhesion Autoimmune diseases
Antibodies Organ systems
Antibody Multiple sclerosis
Antigens Neuromyelitis optica
Assay Medical specialties
Atomic Force Microscopy Aquaporin 4
CBA Medicine
Epitopes Anti-AQP4 disease
Force Antibodies
Multiple Sclerosis
Neuromyelitis Optica
Peptide
Peptides
Phenotypes
Sci
Seronegative
Seropositive
Spectrum

Semantics

Type Source Name
disease MESH diagnosis
disease MESH neuromyelitis optica spectrum disorders
gene UNIPROT AQP4
gene UNIPROT SET
disease MESH multiple sclerosis
disease DOID multiple sclerosis
disease DOID CBA
drug DRUGBANK Coenzyme M
disease DOID neuromyelitis optica
disease MESH syndromes
disease MESH myelitis
disease DOID myelitis
disease MESH optic neuritis
disease DOID optic neuritis
disease MESH inflammation
disease MESH demyelination
gene UNIPROT NT5DC3
gene UNIPROT TTC41P
disease MESH Development
disease MESH dif
gene UNIPROT AFM
gene UNIPROT PTPN22
gene UNIPROT PREP
drug DRUGBANK Piroxicam
gene UNIPROT TNIP1
gene UNIPROT GOPC
gene UNIPROT DEPP1
gene UNIPROT TIPRL
gene UNIPROT ITFG1
gene UNIPROT SGSM3
gene UNIPROT IK
gene UNIPROT SPEN
gene UNIPROT PCBD1
disease MESH separation
drug DRUGBANK Saquinavir
gene UNIPROT ARID3A
gene UNIPROT BFAR
gene UNIPROT NR1H4
gene UNIPROT TBPL1
drug DRUGBANK Indoleacetic acid
gene UNIPROT MOG
disease MESH acute disseminated encephalomyelitis
disease DOID acute disseminated encephalomyelitis
disease MESH myasthenia gravis
disease DOID myasthenia gravis
drug DRUGBANK Acetylcholine
gene UNIPROT MUSK
gene UNIPROT LRP4
drug DRUGBANK Amino acids
drug DRUGBANK Silicon
gene UNIPROT POLR1C
gene UNIPROT MICA
drug DRUGBANK Ozone
drug DRUGBANK Azelaic acid
drug DRUGBANK Polyethylene glycol
gene UNIPROT PAEP
gene UNIPROT MCOLN1
gene UNIPROT SPG7
gene UNIPROT NR1I3
gene UNIPROT CASR
gene UNIPROT CXADR
gene UNIPROT ARR3
gene UNIPROT NHS
gene UNIPROT FAM155B
drug DRUGBANK Water
gene UNIPROT PHEX
gene UNIPROT TACR1
gene UNIPROT SPR
drug DRUGBANK Gold
drug DRUGBANK Cysteamine
gene UNIPROT PTPN5
drug DRUGBANK L-Cysteine
gene UNIPROT DST
pathway BSID Spinal Cord Injury
gene UNIPROT BTG3
disease DOID syndrome
disease MESH neurologic disorders
gene UNIPROT SON
gene UNIPROT UBXN11
gene UNIPROT SIRPA
gene UNIPROT NAB2
gene UNIPROT PDLIM7
disease MESH autoimmune disease
disease DOID autoimmune disease
disease MESH multi
gene UNIPROT PLA2G15
gene UNIPROT ACSS2
gene UNIPROT APPL1
gene UNIPROT NLRP5
drug DRUGBANK Activated charcoal
gene UNIPROT ENG
gene UNIPROT TNFSF14
drug DRUGBANK Dextran
gene UNIPROT STUB1
drug DRUGBANK Efavirenz
pathway BSID Reproduction

Original Article

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