Investigating the epi-miRNome: identification of epi-miRNAs using transfection experiments.

Investigating the epi-miRNome: identification of epi-miRNAs using transfection experiments.

Publication date: Nov 06, 2019

Aim: Growing evidence shows a strong interplay between post-transcriptional regulation, mediated by miRs and epigenetic regulation. Nevertheless, the number of experimentally validated miRs (called epi-miRs) involved in these regulatory circuitries is still very small. Material & methods: We propose a pipeline to prioritize candidate epi-miRs and to identify potential epigenetic interactors of any given miR starting from miR transfection experiment datasets. Results & conclusion: We identified 34 candidate epi-miRs: 19 of them are known epi-miRs, while 15 are new. Moreover, using an in-house generated gene expression dataset, we experimentally proved that a component of the polycomb-repressive complex 2, the histone methyltransferase enhancer of zeste homolog 2 (EZH2), interacts with miR-214, a well-known prometastatic miR in melanoma and breast cancer, highlighting a miR-214-EZH2 regulatory axis potentially relevant in tumor progression.

Reale, E., Taverna, D., Cantini, L., Martignetti, L., Osella, M., Pitt`a, C., Virga, F., Orso, F., and Caselle, M. Investigating the epi-miRNome: identification of epi-miRNAs using transfection experiments. 24691. 2019 Epigenomics.

Concepts Keywords
Aim Branches of biology
Breast Cancer Protein families
Enhancer EZH2
Epigenetic MicroRNA
Epigenomics Polycomb-group proteins
EZH2 Epigenetics
Histone Methyltransferase EED
Homolog Epigenomics
Interplay
Melanoma
Pipeline
Polycomb
Transcriptional Regulation
Transfection
Tumor

Semantics

Type Source Name
gene UNIPROT TFPI
gene UNIPROT CD69
gene UNIPROT DNMT1
gene UNIPROT DESI1
gene UNIPROT SLC35G1
gene UNIPROT MARCH8
gene UNIPROT MYLIP
gene UNIPROT MLXIP
pathway BSID Gene Expression
gene UNIPROT EZH2
disease MESH melanoma
disease DOID melanoma
pathway BSID Melanoma
disease MESH breast cancer
disease DOID breast cancer
pathway BSID Breast cancer
disease MESH tumor

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