NG2 as an Identity and Quality Marker of Mesenchymal Stem Cell Extracellular Vesicles.

NG2 as an Identity and Quality Marker of Mesenchymal Stem Cell Extracellular Vesicles.

Publication date: Nov 27, 2019

The therapeutic potential of mesenchymal stem cell (MSC) extracellular vesicles (EV) is currently under investigation in many pathological contexts. Both adult and perinatal MSC are being considered as sources of EV. Herein, we address antigen expression of cord blood and bone marrow MSC and released EV to define an identity and quality parameter of MSC EV as a medicinal product in the context of clinical applications. The research focuses on EV-shuttled neural/glial antigen 2 (NG2), which has previously been detected as a promising surface marker to distinguish perinatal versus adult MSC. Indeed, NG2 was significantly more abundant in cord blood than bone marrow MSC and MSC EV. Ultracentrifuge-isolated EV were then challenged for their pro-angiogenic properties on an xCELLigence system as quality control. NG2 cord blood MSC EV, but not bone marrow MSC EV, promote bFGF and PDGF-AA proliferative effect on endothelial cells. Likewise, they successfully rescue angiostatin-induced endothelial cell growth arrest. In both cases, the effects are NG2-dependent. These results point at NG2 as an identity and quality parameter for cord blood MSC EV, paving the way for their clinical translation.

Barilani, M., , Peli, Cherubini, A., Dossena, M., , Dolo, and Lazzari, L. NG2 as an Identity and Quality Marker of Mesenchymal Stem Cell Extracellular Vesicles. 24940. 2019 Cells (8):12.

Concepts Keywords
Angiogenic MCSP melanoma
Antigen Branches of biology
BFGF Vesicles
Bone Marrow Cell biology
Clinical Translation Proteoglycans
Cord Blood NG2
Endothelial CSPG4
Endothelial Cells Chondroitin sulfate proteoglycan
Extracellular Vesicles Exosome
Glial Microvesicles
Medicinal Product Extracellular vesicle
Mesenchymal MCSP
PDGF Bone
Perinatal
Ultracentrifuge

Semantics

Type Source Name
drug DRUGBANK Basic Fibroblast Growth Factor
pathway REACTOME Translation
drug DRUGBANK Chondroitin sulfate
disease MESH melanoma
pathway KEGG Melanoma

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