Analysis of Trinucleotide Repeat Stability by Integration at a Chromosomal Ectopic Site.

Publication date: Oct 06, 2019

Expansions of CNG microsatellite tracts are responsible for several neurodegenerative diseases, including myotonic dystrophy type 1, Huntington disease, and spinocerebellar ataxia type 8. Here we show that expanded (CNG) repeats are susceptible not only to expansions and contractions, but are prone to DNA double strand breaks following replication stress. We describe a general strategy for the construction of clonal cell lines containing CNG repeats of various lengths, in which the microsatellites are integrated using the yeast FLP recombinase at a single ectopic recombination acceptor site in the HeLa genome. We illustrate two types of (CTG/CAG) cell lines, one of which contains dual fluorescent marker genes flanking the (CTG/CAG) repeat, and one which does not. We show that long CNG repeats are prone to DNA double strand breaks (DSBs) upon exposure of these cell lines to prolonged replication stress.

Gadgil, R.Y., Rider, S.D., Lewis, T., Barthelemy, J., and Leffak, M. Analysis of Trinucleotide Repeat Stability by Integration at a Chromosomal Ectopic Site. 06861. 2019 Methods Mol Biol (2056):

Concepts Keywords
Acceptor Neurodegeneration
Clonal Recombinase
CNG Spinocerebellar ataxia
Fluorescent Marker Myotonic dystrophy
Genome Trinucleotide repeat disorder
HeLa Mutation
Huntington Microsatellite
Lengths Molecular biology
Microsatellite Genetics
Microsatellites Autosomal dominant disorders
Myotonic Dystrophy Branches of biology
Neurodegenerative Diseases Recombination
Spinocerebellar Ataxia
Yeast

Semantics

Type Source Name
drug DRUGBANK Ganciclovir
drug DRUGBANK Flurbiprofen
disease MESH ataxia
pathway KEGG Huntington disease
disease MESH Huntington disease
disease MESH myotonic dystrophy
pathway REACTOME Neurodegenerative Diseases
disease MESH neurodegenerative diseases

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