Publication date: Jan 09, 2020
Melanoma patients’ plasma contains exosomes produced by malignant and normal cells. Plasma exosomes were isolated and separated by immunocapture into two fractions: melanoma cell-derived exosomes (MTEX) and normal cell-derived exosomes (non-MTEX). Immunosuppressive effects of MTEX on primary human immune cells were evaluated. Exosomes were isolated from plasma of 12 melanoma patients and six healthy donors (HDs). Expression levels of 19 immunoregulatory proteins in MTEX, non-MTEX and HDs exosomes were evaluated by on-bead flow cytometry. Functional/phenotypic changes induced in CD8 T or natural killer (NK) cells by MTEX or non-MTEX were compared. Plasma protein levels were higher in patients than HDs (P?
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Sharma, P., Diergaarde, B., Ferrone, S., Kirkwood, J.M., and Whiteside, T.L. Melanoma cell-derived exosomes in plasma of melanoma patients suppress functions of immune effector cells. 25437. 2020 Sci Rep (10):1.
|Flow Cytometry||Clinical medicine|
|Natural Killer||Branches of biology|
|Phenotypic||Exosomes plasma melanoma|
|drug||DRUGBANK||Sodium lauryl sulfate|
|pathway||KEGG||Acute myeloid leukemia|