Melanoma cell-derived exosomes in plasma of melanoma patients suppress functions of immune effector cells.

Publication date: Jan 09, 2020

Melanoma patients’ plasma contains exosomes produced by malignant and normal cells. Plasma exosomes were isolated and separated by immunocapture into two fractions: melanoma cell-derived exosomes (MTEX) and normal cell-derived exosomes (non-MTEX). Immunosuppressive effects of MTEX on primary human immune cells were evaluated. Exosomes were isolated from plasma of 12 melanoma patients and six healthy donors (HDs). Expression levels of 19 immunoregulatory proteins in MTEX, non-MTEX and HDs exosomes were evaluated by on-bead flow cytometry. Functional/phenotypic changes induced in CD8 T or natural killer (NK) cells by MTEX or non-MTEX were compared. Plasma protein levels were higher in patients than HDs (P?

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Sharma, P., Diergaarde, B., Ferrone, S., Kirkwood, J.M., and Whiteside, T.L. Melanoma cell-derived exosomes in plasma of melanoma patients suppress functions of immune effector cells. 25437. 2020 Sci Rep (10):1.

Concepts Keywords
Bead Immunotherapy
CD8 Exosome
Exosomes Melanoma
Flow Cytometry Clinical medicine
Malignant Vesicles
Melanoma Medicine
Natural Killer Branches of biology
Phenotypic Exosomes plasma melanoma
Plasma
Sci

Semantics

Type Source Name
disease MESH tumor escape
disease MESH tumor
pathway KEGG Apoptosis
drug DRUGBANK Coenzyme M
disease MESH separated
drug DRUGBANK Biotin
drug DRUGBANK Sodium lauryl sulfate
disease MESH Pathology
drug DRUGBANK Honey
pathway KEGG Melanoma
disease MESH Melanoma
disease MESH recurrence
disease MESH diagnosis
disease MESH death
disease MESH multiple
drug DRUGBANK Isoxaflutole
drug DRUGBANK Spinosad
drug DRUGBANK Methionine
drug DRUGBANK Gold
drug DRUGBANK Chondroitin sulfate
disease MESH Neck Cancer
pathway KEGG Acute myeloid leukemia
disease MESH growth
disease MESH Metastasis
disease MESH immune disease
drug DRUGBANK Cetuximab
drug DRUGBANK Ipilimumab

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