Integrated transcriptome expression profiling reveals a novel lncRNA associated with L-DOPA-induced dyskinesia in a rat model of Parkinson’s disease.

Publication date: Jan 10, 2020

Levodopa-induced dyskinesia (LID) is a common complication of chronic dopamine replacement therapy in the treatment of Parkinson’s disease (PD). Long noncoding RNAs regulate gene expression and participate in many biological processes. However, the role of long noncoding RNAs in LID is not well understood. In the present study, we examined the lncRNA transcriptome profile of a rat model of PD and LID by RNA sequence and got a subset of lncRNAs, which were gradually decreased during the development of PD and LID. We further identified a previously uncharacterized long noncoding RNA, NONRATT023402.2, and its target genes glutathione S-transferase omega (Gsto)2 and prostaglandin E receptor (Ptger)3. All of them were decreased in the PD and LID rats as shown by quantitative real-time PCR, fluorescence in situ hybridization and western blotting. Pearson’s correlation analysis showed that their expression was positively correlated with the dyskinesia score of LID rats. In vitro experiments by small interfering RNA confirmed that slicing NONRATT023402 inhibited Gsto2 and Ptger3 and promoted the inflammatory response. These results demonstrate that NONRATT023402.2 may have inhibitive effects on the development of PD and LID.

Han, C.L., Liu, Y.P., Sui, Y.P., Chen, N., Du, T.T., Jiang, Y., Guo, C.J., Wang, K.L., Wang, Q., Fan, S.Y., Shimabukuro, M., Meng, F.G., Yuan, F., and Zhang, J.G. Integrated transcriptome expression profiling reveals a novel lncRNA associated with L-DOPA-induced dyskinesia in a rat model of Parkinson’s disease. 23626. 2020 Aging (Albany NY) (12):

Concepts Keywords
Albany Hybridization
Correlation Transcriptome
DOPA L-DOPA
Dopamine Non-coding DNA
Dyskinesia Levodopa-induced dyskinesia
Expression Profiling Long non-coding RNA
Fluorescence Gene expression
Glutathione Parkinson’s disease
Hybridization RNA
Inflammatory Response Nucleic acids
Levodopa Branches of biology
LncRNA
LncRNAs
Long Noncoding RNA
Parkinson
Real Time PCR
Transcriptome
Transferase
Western Blotting

Semantics

Type Source Name
drug DRUGBANK Levodopa
disease MESH dyskinesia
drug DRUGBANK Dopamine
disease MESH development
drug DRUGBANK Glutathione

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