Reprint of: Manipulation of microbiota reveals altered callosal myelination and white matter plasticity in a model of Huntington disease.

Publication date: Jan 10, 2020

Structural and molecular myelination deficits represent early pathological features of Huntington disease (HD). Recent evidence from germ-free (GF) animals suggests a role for microbiota-gut-brain bidirectional communication in the regulation of myelination. In this study, we aimed to investigate the impact of microbiota on myelin plasticity and oligodendroglial population dynamics in the mixed-sex BACHD mouse model of HD. Ultrastructural analysis of myelin in the corpus callosum revealed alterations of myelin thickness in BACHD GF compared to specific-pathogen free (SPF) mice, whereas no differences were observed between wild-type (WT) groups. In contrast, myelin compaction was altered in all groups when compared to WT SPF animals. Levels of myelin-related proteins were generally reduced, and the number of mature oligodendrocytes was decreased in the prefrontal cortex under GF compared to SPF conditions, regardless of genotype. Minor differences in commensal bacteria at the family and genera levels were found in the gut microbiota of BACHD and WT animals housed in standard living conditions. Our findings indicate complex effects of a germ-free status on myelin-related characteristics, and highlight the adaptive properties of myelination as a result of environmental manipulation.

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Radulescu, C.I., Garcia-Miralles, M., Sidik, H., Bardile, C.F., Yusof, N.A.B.M., Lee, H.U., Ho, E.X.P., Chu, C.W., Layton, E., Low, D., De Sessions, P.F., Pettersson, S., Ginhoux, F., and Pouladi, M.A. Reprint of: Manipulation of microbiota reveals altered callosal myelination and white matter plasticity in a model of Huntington disease. 06878. 2020 Neurobiol Dis.

Concepts Keywords
Bacteria Genotype
Brain Human gastrointestinal microbiota
Commensal Corpus callosum
Corpus Callosum Myelin
Genotype Oligodendrocyte
Gut Microbiota Glial cells
Huntington Firmicutes
Mice Environmental microbiology
Microbiota Microbiology
Myelin Bacteriology
Myelination Branches of biology
Oligodendrocytes Microbiomes
Population Dynamics
Prefrontal Cortex
White Matter
Wild Type


Type Source Name
disease MESH Huntington disease
pathway KEGG Huntington disease
disease MESH Dementia
drug DRUGBANK Glutathione
disease MESH growth
drug DRUGBANK Pyridoxal Phosphate
disease MESH neurodegenerative disorder
drug DRUGBANK Adenine
drug DRUGBANK Guanine
disease MESH pathology
disease MESH atrophy
disease MESH defects
drug DRUGBANK Medical air
drug DRUGBANK Water
drug DRUGBANK Tretamine
drug DRUGBANK Ketamine
drug DRUGBANK Xylazine
drug DRUGBANK Phosphate ion
drug DRUGBANK Sucrose
disease MESH dehydration
drug DRUGBANK Ethanol
drug DRUGBANK Sodium Citrate
drug DRUGBANK Nitrogen
drug DRUGBANK Thiothixene
drug DRUGBANK Tromethamine
drug DRUGBANK Abacavir
drug DRUGBANK Coenzyme M
disease MESH community
disease MESH multiple

Original Article

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