Up-regulation of microRNA-375 ameliorates the damage of dopaminergic neurons, reduces oxidative stress and inflammation in Parkinson’s disease by inhibiting SP1.

Publication date: Jan 11, 2020

This study is conducted to investigate the protective role of elevated microRNA-375 (miR-375) in dopaminergic neurons in Parkinson’s disease through down-regulating transcription factor specificity protein 1 (SP1).

The successfully modeled rats with Parkinson’s disease showed aggregated neurobehavioral change, increased neuroinflammatory response and oxidative stress, and lowered dopamine content. Parkinson’s disease rats treated with overexpressed miR-375 displayed improved neurobehavioral change, ameliorated neuroinflammatory response and oxidative stress, heightened dopamine content and abated neuronal apoptosis by down-regulating SP1. Up-regulation of SP1 reversed the protective effect of upregulated miR-375 on Parkinson’s disease.

Up-regulation of miR-375 ameliorated the damage of dopaminergic neurons, reduced oxidative stress and inflammation in Parkinson’s disease by inhibiting SP1.

Parkinson’s disease rat model was established by targeted injection of 6-hydroxydopamine to damage the substantia nigra striatum. The successfully modeled Parkinson’s disease rats were intracerebroventricularly injected with miR-375 mimics or pcDNA3.1-SP1. The functions of miR-375 and SP1 in neurobehavioral change, neuroinflammatory response, oxidative stress, dopamine content and expression of apoptosis-related proteins in the substantia nigra of Parkinson’s disease rats were evaluated. The target relation of miR-375 and SP1 was confirmed by bioinformatics analysis and dual luciferase reporter gene assay.

Cai, L.J., Tu, L., Li, T., Yang, X.L., Ren, Y.P., Gu, R., Zhang, Q., Yao, H., Qu, X., Wang, Q., and Tian, J.Y. Up-regulation of microRNA-375 ameliorates the damage of dopaminergic neurons, reduces oxidative stress and inflammation in Parkinson’s disease by inhibiting SP1. 23620. 2020 Aging (Albany NY) (12):

Concepts Keywords
Albany Branches of biology
Apoptosis Brain
Assay Drugs
Dopamine Basal ganglia
Dopaminergic Midbrain
Inflammation Gene expression
Luciferase RNA
MicroRNA Amphetamine
Neurons MicroRNA
Oxidative Stress Substantia nigra
Parkinson Dopamine
Striatum Neuron
Substantia Nigra Apoptosis
Transcription Factor

Semantics

Type Source Name
disease MESH oxidative stress
disease MESH inflammation
drug DRUGBANK Dopamine
pathway KEGG Apoptosis

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