Sanggenol L promotes apoptotic cell death in melanoma skin cancer cells through activation of caspase cascades and apoptosis-inducing factor.

Publication date: Feb 18, 2020

Sanggenol L is one component of root bark of Morus alba. The molecular and cellular mechanisms of sanggenol L effects on melanoma cells are not well known. Recently, melanoma is the most common skin cancer with a high mortality rate not only in United States, but also in East Asia. Therefore, safe and effective treatments for melanoma treatment are required. In this study, we investigated whether or not sanggenol L possesses anti-cancer activity in human and mouse melanoma skin cancer cells. Sanggenol L treatment exerted significant cell growth inhibitory effects and inhibited colony formation capacity against B16, SK-MEL-2, and SK-MEL-28 melanoma skin cancer cells, whereas HaCaT human epithelial keratinocyte cells was unaffected by sanggenol L treatment. Sanggenol L treatment resulted in apoptotic cell death in melanoma skin cancer cells, which was characterized by accumulation of apoptotic cells, nuclear condensation, and apoptotic bodies. We also showed that sanggenol L treatment induced caspase-dependent apoptosis (up-regulation of Bax and cleaved-PARP or down-regulation of Bid, Bcl-2, procaspse-3, -8, and -9), induction of caspase-independent apoptosis (up-regulation of AIF and Endo G on cytosol) in melanoma skin cancer cells. These results suggest that sanggenol L induces caspase-dependent and -independent apoptosis in melanoma skin cancer cells.

Won, Y.S. and Seo, K.I. Sanggenol L promotes apoptotic cell death in melanoma skin cancer cells through activation of caspase cascades and apoptosis-inducing factor. 25890. 2020 Food Chem Toxicol.

Concepts Keywords
Apoptosis Melanoma treatment
Apoptotic Apoptotic death melanoma
Apoptotic Cells Branches of biology
Bark Cell biology
Bax Programmed cell death
Cancer Cellular processes
Caspase Apoptosis
Colony Caspase
Condensation Bcl-2
Cytosol Mensacarcin
East Asia Condensation
Epithelial AIF
Keratinocyte Apoptosis
MEL
Melanoma
Mortality Rate
Morus Alba
PARP
United States

Semantics

Type Source Name
disease MESH melanoma
pathway KEGG Melanoma
disease MESH skin cancer
pathway KEGG Apoptosis
disease MESH cancer
drug DRUGBANK Honey

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