Curcumin, demethoxycurcumin, and bisdemethoxycurcumin induced caspase-dependent and -independent apoptosis via Smad or Akt signaling pathways in HOS cells.

Publication date: Mar 03, 2020

Osteosarcoma is the most common primary malignant bone tumor in children and adolescents and has also been associated with a high degree of malignancy and enhanced metastatic capacity. Curcumin (CUR) is well known for its anti-osteosarcoma activity. However, both demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC) are natural curcumin analogues/congeners from turmeric whose role in osteosarcoma development remains unknown.

To evaluate the growth inhibitory effects of CUR, DMC and BDMC on osteosarcoma (HOS and U2OS), breast (MDA-MB-231), and melanoma (A2058) cancer cells, we employed the MTT assay, annexin V-FITC /7-AAD staining, and clonogenic assay.

CUR,DMC, and BDMC all decreased the viability of HOS, U2OS, MDA-MB-231, and A2058 cancer cells. Additionally, CUR,DMC, and BDMC induced the apoptosis of HOS cells through activation of Smad 2/3 or repression of Akt signaling pathway. Furthermore, the combination of CUR,DMC, and BDMC synergistically reduced cell viability, colony formation and increased apoptosis than either two or a single agent in HOS cells.

The combination of these three compounds could be used as a novel target for the treatment of osteosarcoma.

Open Access PDF

Huang, C., Lu, H.F., Chen, Y.H., Chen, J.C., Chou, W.H., and Huang, H.C. Curcumin, demethoxycurcumin, and bisdemethoxycurcumin induced caspase-dependent and -independent apoptosis via Smad or Akt signaling pathways in HOS cells. 26027. 2020 BMC Complement Med Ther (20):1.

Concepts Keywords
Akt Programmed cell death
Apoptosis Osteosarcoma
BMC Immunology
Bone Tumor Cellular senescence
Breast Cell signaling
Caspase Peripheral membrane proteins
Colony Apoptosis
Congeners Branches of biology
Curcumin Assay
HOS Protein kinase B
Malignancy Annexin A5
Malignant Apoptosis
MB
MDA
Melanoma
Metastatic
Osteosarcoma
Smad
Staining
The Combination
Turmeric

Semantics

Type Source Name
drug DRUGBANK Curcumin
pathway KEGG Apoptosis
disease MESH Osteosarcoma
disease MESH tumor
disease MESH development
disease MESH melanoma
pathway KEGG Melanoma
disease MESH repression

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