Modeling the complex genetic architectures of brain disease.

Publication date: Mar 23, 2020

The genetic architecture of each individual comprises common and rare variants that, acting alone and in combination, confer risk of disease. The cell-type-specific and/or context-dependent functional consequences of the risk variants linked to brain disease must be resolved. Coupling human induced pluripotent stem cell (hiPSC)-based technology with CRISPR-based genome engineering facilitates precise isogenic comparisons of variants across genetic backgrounds. Although functional-validation studies are typically performed on one variant in isolation and in one cell type at a time, complex genetic diseases require multiplexed gene perturbations to interrogate combinations of genes and resolve physiologically relevant disease biology. Our aim is to discuss advances at the intersection of genomics, hiPSCs and CRISPR. A better understanding of the molecular mechanisms underlying disease risk will improve genetic diagnosis, drive phenotypic drug discovery and pave the way toward precision medicine.

Fernando, M.B., Ahfeldt, T., and Brennand, K.J. Modeling the complex genetic architectures of brain disease. 06555. 2020 Nat Genet.

Concepts Keywords
Brain Complex genetic diseases
Cell Type Risk disease
Coupling Architectures brain disease
CRISPR Branches of biology
Genetic Life sciences
Genetic Diseases Biotechnology
Genome Engineering Molecular biology
Genomics Biological engineering
Isogenic Emerging technologies
Multiplexed Genetics
Perturbations Genome editing
Phenotypic CRISPR
Pluripotent Genetic architecture
Precision Medicine Human Nature


Type Source Name
disease MESH brain disease
disease MESH genetic diseases
drug DRUGBANK Tropicamide
disease MESH diagnosis


Original Article

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