[F]-DPA-714 PET as a specific in vivo marker of early microglial activation in a rat model of progressive dopaminergic degeneration.

[F]-DPA-714 PET as a specific in vivo marker of early microglial activation in a rat model of progressive dopaminergic degeneration.

Publication date: Mar 23, 2020

To study the feasibility of the in vivo [F]-DPA-714 TSPO positron emission tomography (PET) to detect glial activation in a rat model of progressive parkinsonism induced by viral-mediated overexpression of A53T mutated human ?-synuclein (h?-syn) in the substantia nigra pars compacta (SNpc).

We conducted a cross-sectional study in a model of progressive parkinsonism. Bilateral intranigral injections with 2/9 adeno-associated viral vectors encoding either h?-syn (AAV-h?-syn) or green fluorescent protein (AAV-GFP) were performed in rats (n?=?60). In vivo [F]-DPA-714 PET imaging was performed at different time points after inoculation (p.i.) of the viral vector (24 and 72 h and 1, 2, 3, and 16 weeks). Images were analyzed to compute values of binding potential (BP) in the SNpc and striatum using a volume of interest (VOI) analysis. Immunohistochemistry of markers of dopaminergic degeneration (tyrosine hydroxylase (TH)), microglia (Iba-1), and astrocytes (GFAP) was carried out. Binding potential (BP) of [F]-DPA-714 PET in the in vivo PET study was correlated with post-mortem histological markers.

In the SNpc of AAV-h?-syn rats, there was higher in vivo [F]-DPA-714 BP (p?

Rodr’iguez-Chinchilla, T., Quiroga-Varela, A., Molinet-Dronda, F., Belloso-Iguerategui, A., Merino-Galan, L., Jimenez-Urbieta, H., Gago, B., and Rodriguez-Oroz, M.C. -DPA-714 PET as a specific in vivo marker of early microglial activation in a rat model of progressive dopaminergic degeneration. 24452. 2020 Eur J Nucl Med Mol Imaging.

Concepts Keywords
Astrocytes Imaging
BP Branches of biology
Cross Basal ganglia
Dopaminergic Midbrain
DPA Organ systems
Fluorescent Nervous system
GFAP Translocator protein
GFP Substantia nigra
Glial Pars compacta
Histological Parkinson’s disease
Immunohistochemistry Neuroinflammation
Inoculation Microglia
Microglia Tomography
Parkinsonism
PET
PET Imaging
Positron Emission Tomography
Post Mortem
Progressive
Striatum
Tyrosine Hydroxylase
Viral
Viral Vector
Viral Vectors

Semantics

Type Source Name
drug DRUGBANK Valproic Acid
drug DRUGBANK L-Tyrosine

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