Mitochondrial ATPase inhibitor factor 1, MjATPIF1, is beneficial for WSSV replication in kuruma shrimp (Marsupenaeus japonicus).

Publication date: Mar 01, 2020

ATPase Inhibitory Factor 1 (IF1) is a mitochondrial protein that functions as a physiological inhibitor of FF-ATP synthase. In the present study, a mitochondrial ATPase inhibitor factor 1 (MjATPIF1) was identified from kuruma shrimp (Marsupenaeus japonicus), which was demonstrated to participate in the viral immune reaction of white spot syndrome virus (WSSV). MjATPIF1 contained a mitochondrial ATPase inhibitor (IATP) domain, and was widely distributed in hemocytes, heart, hepatopancreas, gills, stomach, and intestine of shrimp. MjATPIF1 transcription was upregulated in hemocytes and intestines by WSSV. WSSV replication decreased after MjATPIF1 knockdown by RNA interference and increased following recombinant MjATPIF1 protein injection. Further study found that MjATPIF1 promoted the production of superoxide and activated the transcription factor nuclear factor kappa B (NF-?B, Dorsal) to induce the transcription of WSSV RNAs. These results demonstrate that MjATPIF1 benefits WSSV replication in kuruma shrimp by inducing superoxide production and NF-?B activation.

Concepts Keywords
ATP Synthase Aquaculture
ATPase Animal virology
Gills Nimaviridae
Hemocytes Penaeidae
Hepatopancreas White spot syndrome
Immune Reaction Protein families
Inhibitor Kuruma
Intestine ATPIF1
Intestines Branches of biology
Mitochondrial Health
Recombinant Organisms
Shrimp
Stomach
Superoxide
Transcription
Transcription Factor
Viral

Semantics

Type Source Name
drug DRUGBANK ATP

Original Article

Leave a Comment

Your email address will not be published. Required fields are marked *