Selective Naked-Eye Detection of SARS-CoV-2 Mediated by N Gene Targeted Antisense Oligonucleotide Capped Plasmonic Nanoparticles.

Selective Naked-Eye Detection of SARS-CoV-2 Mediated by N Gene Targeted Antisense Oligonucleotide Capped Plasmonic Nanoparticles.

Publication date: May 21, 2020

The current outbreak of the pandemic coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) demands its rapid, convenient, and large-scale diagnosis to downregulate its spread within as well as across the communities. But the reliability, reproducibility, and selectivity of majority of such diagnostic tests fail when they are tested either to a viral load at its early representation or to a viral gene mutated during its current spread. In this regard, a selective ‘naked-eye’ detection of SARS-CoV-2 is highly desirable, which can be tested without accessing any advanced instrumental techniques. We herein report the development of a colorimetric assay based on gold nanoparticles (AuNPs), those when capped with suitably designed thiol modified antisense oligonucleotides (ASOs) specific for N-gene (nucleocapsid phosphoprotein) of SARS-CoV-2, could be used for diagnosing positive COVID-19 cases within 10 minutes from the isolated RNA samples. The thiol modified ASO capped AuNPs agglomerate selectively in the presence of its target RNA sequence of SARS-CoV-2 and demonstrate a change in its surface plasmon resonance (SPR). Further, the addition of RNaseH cleaves the RNA strand from the RNA-DNA hybrid leading to a visually detectable precipitate from the solution mediated by the additional agglomeration among the AuNPs. The selectivity of the assay has been monitored in presence of MERS-CoV viral RNA with a limit of detection of 0.18 ng/uL of RNA having SARS-CoV-2 viral load. Thus, the current study reports a selective, and visual ‘naked-eye’ detection of COVID-19 causative virus, SARS-CoV-2, without the requirement of any sophisticated instrumental techniques.

Concepts Keywords
Agglomerate Diagnostic tests
Agglomeration Colloidal gold
Antisense In Saudi Arabia
ASOs Animal virology
Assay Zoonoses
Causative Viruses
Coronavirus Nidovirales
DNA
Downregulate
Gene
Gold
Hybrid
MERS
Naked Eye
Nanoparticles
Nucleocapsid
Oligonucleotide
Oligonucleotides
Pandemic
Phosphoprotein
Precipitate
Reproducibility
RNA
RNaseH
SARS
Surface Plasmon Resonance
Thiol
Viral
Viral Load
Virus

Semantics

Type Source Name
disease MESH severe acute respiratory syndrome
disease MESH diagnosis
disease MESH communities
disease MESH viral load
disease MESH development
drug DRUGBANK Gold
disease MESH visual

Original Article

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