Profiling GABA(A) Receptor Subunit Expression in the Hippocampus of PMDD Rat Models Based on TCM Theories.

Publication date: Aug 01, 2023

γ-Aminobutyric acid type A receptors (GABAARs) play an important role in cognitive and emotional regulation and are related to the hippocampus. However, little is known regarding patterns of hippocampal GABAAR subunit expression in rat models of premenstrual dysphoric disorder (PMDD). This study investigated the above changes by establishing two PMDD rat models based on Traditional Chinese Medicine (TCM) theories, namely, PMDD liver-qi invasion syndrome (PMDD-LIS) and PMDD liver-qi depression syndrome (PMDD-LDS). Behavioral tests were used to detect depression and irritability emotion. Western blot analysis was used to investigate protein levels of GABAAR α1, α2, α4, α5, β2, β3, and δ subunits, whereas ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analysis was performed to determine gamma-aminobutyric acid (GABA) and glutamate (Glu) levels in the hippocampus across each group. Concurrently, behavioral data indicated that the PMDD-LDS and PMDD-LIS rat models had been successfully established. GABAAR α2, α5, β2, and δ subunit was significantly upregulated, whereas α4 was significantly downregulated (P 

Concepts Keywords
Chinese Animals
Dysphoric Depression
Hippocampus Female
Liver GABAA receptor
Spectrometry gamma-Aminobutyric Acid
gamma-Aminobutyric Acid
Medicine, Chinese Traditional
Premenstrual Dysphoric Disorder
Premenstrual dysphoric disorder
Rat model
Rats, Sprague-Dawley
Receptors, GABA-A
Receptors, GABA-A
Tandem Mass Spectrometry
TCM theories


Type Source Name
drug DRUGBANK gamma-Aminobutyric acid
disease MESH premenstrual dysphoric disorder
disease MESH syndrome
drug DRUGBANK Glutamic Acid

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