Publication date: Jun 29, 2023
The hydroxyapatite-lanthanum strontium cobalt ferrite (HA-LSCF) composite showed a good response on a screen-printed carbon electrode (SPCE) electrochemical aptasensor to detect SARS-CoV-2. SPCE/HA-LSCF with a thiolated aptamer has a strong affinity for the SARS-CoV-2 spike RBD protein. This occurs due to the binding of -SH to the HA-positive region. In the presence of LSCF, which is conductive, an increase in electron transfer from the redox system [Fe(CN)] occurs. The interaction of the aptamer with the RBD protein can be observed based on the decrease in the electron transfer process. As a result, the developed biosensor is highly sensitive to the SARS-CoV-2 spike RBD protein with a linear range of 0. 125 to 2. 0 ng mL, a detection limit of 0. 012 ng mL, and a quantification limit of 0. 040 ng mL. The analytical application of the aptasensor demonstrates its feasibility in the analysis of saliva or swab samples.
| Concepts | Keywords |
|---|---|
| Biosensor | Aptamer |
| Decrease | Aptasensor |
| Hydroxyapatite | Cobalt |
| Strong | Cov |
| Strontium | Electrochemical |
| Ferrite | |
| Hydroxyapatite | |
| Lanthanum | |
| Lscf | |
| Ml | |
| Rbd | |
| Sars | |
| Spce | |
| Spike | |
| Strontium |
Semantics
| Type | Source | Name |
|---|---|---|
| drug | DRUGBANK | Cobalt |
| drug | DRUGBANK | Activated charcoal |
| disease | IDO | process |