Publication date: Jul 08, 2023
The utility of reverse transcription-polymerase chain reaction (RT-PCR) in analysis SARS-COV-2 variants was evaluated. RT-PCR tests were used to analyse the majority of new SARS-CoV-2 cases (n = 9315) in a tertiary hospital (Madrid, Spain) throughout 2021. Subsequently, whole genome sequencing (WGS) was conducted on 10. 8% of these samples (n = 1002). Notably, the Delta and Omicron variants emerged rapidly. There were no discrepancies between RT-PCR and WGS results. Continuous surveillance of SARS-CoV-2 variants is essential, and RT-PCR is a highly useful method, specially during periods of high COVID-19 incidence. This feasible technique can be implemented in all SARS-CoV-2 laboratories. However, WGS remains the gold standard method for comprehensive detection of all existing SARS-CoV-2 variants.
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| Concepts | Keywords |
|---|---|
| Pandemic | Cov |
| Pcr | Key |
| Spain | Pandemic |
| Pcr | |
| Polymerase | |
| Rapid | |
| Reverse | |
| Rt | |
| Sars | |
| Screening | |
| Surveillance | |
| Transcription | |
| Utility | |
| Variants | |
| Wgs |
Semantics
| Type | Source | Name |
|---|---|---|
| disease | MESH | COVID-19 |
| drug | DRUGBANK | Gold |
| drug | DRUGBANK | Coenzyme M |
| disease | MESH | sid |
| drug | DRUGBANK | Spinosad |
| disease | IDO | susceptibility |
| disease | VO | vaccinated |
| disease | MESH | emergency |
| disease | IDO | process |
| disease | VO | USA |
| drug | DRUGBANK | Lauric Acid |
| disease | VO | frequency |
| disease | IDO | assay |
| drug | DRUGBANK | Etoperidone |
| disease | VO | time |
| disease | VO | gene |
| drug | DRUGBANK | Troleandomycin |
| disease | MESH | clinical significance |
| disease | MESH | immunocompromised patient |
| pathway | REACTOME | Reproduction |