Midgut transcriptomic responses to dengue and chikungunya viruses in the vectors Aedes albopictus and Aedes malayensis.

Publication date: Jul 12, 2023

Dengue (DENV) and chikungunya (CHIKV) viruses are among the most preponderant arboviruses. Although primarily transmitted through the bite of Aedes aegypti mosquitoes, Aedes albopictus and Aedes malayensis are competent vectors and have an impact on arbovirus epidemiology. Here, to fill the gap in our understanding of the molecular interactions between secondary vectors and arboviruses, we used transcriptomics to profile the whole-genome responses of A. albopictus to CHIKV and of A. malayensis to CHIKV and DENV at 1 and 4 days post-infection (dpi) in midguts. In A. albopictus, 1793 and 339 genes were significantly regulated by CHIKV at 1 and 4 dpi, respectively. In A. malayensis, 943 and 222 genes upon CHIKV infection, and 74 and 69 genes upon DENV infection were significantly regulated at 1 and 4 dpi, respectively. We reported 81 genes that were consistently differentially regulated in all the CHIKV-infected conditions, identifying a CHIKV-induced signature. We identified expressed immune genes in both mosquito species, using a de novo assembled midgut transcriptome for A. malayensis, and described the immune architectures. We found the JNK pathway activated in all conditions, generalizing its antiviral function to Aedines. Our comprehensive study provides insight into arbovirus transmission by multiple Aedes vectors.

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Concepts Keywords
4days Aedes
Aegypti Albopictus
Arboviruses Arbovirus
Competent Arboviruses
Transcriptomics Chikungunya


Type Source Name
disease MESH dengue
disease MESH infection
drug DRUGBANK Coenzyme M
disease MESH hemorrhage
disease MESH shock
disease MESH death
disease MESH polyarthralgia
disease MESH arbovirus infections
drug DRUGBANK Spinosad
disease MESH Emerging Infectious Diseases
disease MESH Liver Disease
pathway REACTOME Signal Transduction
drug DRUGBANK Imidacloprid
drug DRUGBANK Serine
drug DRUGBANK Fibrinogen Human
drug DRUGBANK L-Lysine
drug DRUGBANK Oxygen
disease MESH Viral infection
pathway KEGG Autophagy
pathway KEGG Apoptosis
disease MESH viremia
drug DRUGBANK Pidolic Acid
drug DRUGBANK Methionine
drug DRUGBANK L-Tryptophan
disease MESH PRRs
drug DRUGBANK Lysozyme
disease MESH oxidative stress
drug DRUGBANK Pentaerythritol tetranitrate
pathway KEGG Viral replication
pathway KEGG Adherens junction
drug DRUGBANK Water
drug DRUGBANK Sucrose
drug DRUGBANK Cefaclor
drug DRUGBANK Gold
disease MESH Vector borne diseases
drug DRUGBANK Dacarbazine
disease MESH sti
disease MESH chikungunya virus infection
drug DRUGBANK Sulfasalazine

Original Article

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