Publication date: Oct 11, 2024
Melanoma is still one of the deadliest cancers whose prevalence has increased in recent decades. Today, many polysaccharides and their bioactive compounds have been of special importance in modern biotechnology. They have various biological and therapeutic properties. they can regulate and strengthen the immune system, lower blood pressure and cholesterol, and reduce bacterial and viral infections. According to studies, these compounds also have antitumor properties. we investigated the cytotoxic effects of β-Glucan obtained from solid-state fermentation (SSF) of edible medicinal mushroom Lentinus edodes on cancerous skin cells. The mitochondria were isolated from melanoma cells via differential centrifugation and treated with various concentrations (30, 45, 60, 90, 120, and 240 ug/ml) of β-Glucan extract. Then, they were subjected to MTT, ROS, MMP decline, mitochondrial swelling, cytochrome c release, and flow cytometry assays. The results of the MTT assay showed that IC50 of β-Glucan extract was 60 μg/ml, and it induced a selectively significant (P
| Concepts | Keywords |
|---|---|
| Biotechnology | apoptosis |
| Cholesterol | melanoma |
| Deadliest | mitochondria |
| Decades | reactive oxygen species |
| Viral | β-Glucan |
Semantics
| Type | Source | Name |
|---|---|---|
| disease | MESH | melanoma |
| pathway | KEGG | Melanoma |
| disease | MESH | cancers |
| drug | DRUGBANK | Nonoxynol-9 |
| pathway | REACTOME | Immune System |
| drug | DRUGBANK | Cholesterol |
| disease | MESH | viral infections |
| disease | MESH | mitochondrial swelling |
| pathway | REACTOME | Release |
| pathway | REACTOME | Apoptosis |