Publication date: Jan 15, 2025
Binding and neutralizing antibodies are critical indicators of protection against viral pathogens and are essential for assessing the immunogenicity and efficacy of a vaccine. Here, we present a protocol comprising two assays for measuring the spike-specific binding and neutralizing antibodies in mouse plasma following severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccination. We describe steps for determining binding antibody titers using enzyme-linked immunosorbent assay (ELISA) and assessing neutralizing antibody titers through a pseudovirus neutralization assay. For complete details on the use and execution of this protocol, please refer to Jingyou Yu et al. .
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Concepts | Keywords |
---|---|
Complete | cell biology |
Elisa | cell-based assays |
Jingyou | immunology |
Pseudovirus | microbiology |
Vaccine | molecular biology |
Semantics
Type | Source | Name |
---|---|---|
disease | IDO | assay |
disease | IDO | pathogen |
disease | IDO | facility |
disease | IDO | production |
drug | DRUGBANK | Flunarizine |
drug | DRUGBANK | Albendazole |
disease | MESH | infection |
pathway | REACTOME | Digestion |
drug | DRUGBANK | Methylergometrine |
drug | DRUGBANK | Trypsin |
drug | DRUGBANK | Edetic Acid |
disease | IDO | replication |
disease | IDO | process |
disease | MESH | re infection |
disease | MESH | viral infection |
drug | DRUGBANK | Isoxaflutole |
drug | DRUGBANK | Sodium lauryl sulfate |
disease | IDO | protein |
disease | IDO | humoral immune response |
disease | IDO | blood |
drug | DRUGBANK | Sucrose |
drug | DRUGBANK | Tromethamine |
drug | DRUGBANK | Glycerin |
drug | DRUGBANK | Imidazole |
disease | IDO | reagent |
drug | DRUGBANK | Coenzyme M |
drug | DRUGBANK | Deoxycholic Acid |
drug | DRUGBANK | Trestolone |
disease | IDO | immune response |
drug | DRUGBANK | Medical air |
drug | DRUGBANK | Guanosine |
disease | MESH | breakthrough infection |
disease | MESH | SARS CoV 2 infection |
disease | IDO | infectivity |
disease | IDO | cell |
Original Article
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