Protocol for evaluating humoral immune responses in mice following SARS-CoV-2 vaccination.

Protocol for evaluating humoral immune responses in mice following SARS-CoV-2 vaccination.

Publication date: Jan 15, 2025

Binding and neutralizing antibodies are critical indicators of protection against viral pathogens and are essential for assessing the immunogenicity and efficacy of a vaccine. Here, we present a protocol comprising two assays for measuring the spike-specific binding and neutralizing antibodies in mouse plasma following severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccination. We describe steps for determining binding antibody titers using enzyme-linked immunosorbent assay (ELISA) and assessing neutralizing antibody titers through a pseudovirus neutralization assay. For complete details on the use and execution of this protocol, please refer to Jingyou Yu et al. .

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Concepts Keywords
Complete cell biology
Elisa cell-based assays
Jingyou immunology
Pseudovirus microbiology
Vaccine molecular biology

Semantics

Type Source Name
disease IDO assay
disease IDO pathogen
disease IDO facility
disease IDO production
drug DRUGBANK Flunarizine
drug DRUGBANK Albendazole
disease MESH infection
pathway REACTOME Digestion
drug DRUGBANK Methylergometrine
drug DRUGBANK Trypsin
drug DRUGBANK Edetic Acid
disease IDO replication
disease IDO process
disease MESH re infection
disease MESH viral infection
drug DRUGBANK Isoxaflutole
drug DRUGBANK Sodium lauryl sulfate
disease IDO protein
disease IDO humoral immune response
disease IDO blood
drug DRUGBANK Sucrose
drug DRUGBANK Tromethamine
drug DRUGBANK Glycerin
drug DRUGBANK Imidazole
disease IDO reagent
drug DRUGBANK Coenzyme M
drug DRUGBANK Deoxycholic Acid
drug DRUGBANK Trestolone
disease IDO immune response
drug DRUGBANK Medical air
drug DRUGBANK Guanosine
disease MESH breakthrough infection
disease MESH SARS CoV 2 infection
disease IDO infectivity
disease IDO cell

Original Article

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