Dissecting response to neoadjuvant immunotherapy-treated melanoma using cancer-immunity cycle-associated signatures.

Publication date: Jun 25, 2025

Neoadjuvant combination therapy with anti-PD-1 and anti-CTLA4 agents has significantly improved long-term survival in patients with metastatic melanoma, yet not all patients respond to treatment. Responders often exhibit upregulated baseline inflammatory signatures; however, these markers capture only a single facet of the Cancer-Immunity Cycle-a comprehensive model describing the sequential steps required for effective anti-cancer immunity. To determine whether non-responsiveness to immunotherapy arises from single or multiple ‘defective’ steps, we analyzed in melanoma patients, treated with neoadjuvant immunotherapy, gene signatures representing each step of the cycle. Patients not achieving a major pathological response showed overall lower expression of these signatures. Among the ‘immune-hot’ patients, we identified a low response subgroup defective in one step (‘homing-to-the-tumor,’ involving CXCL9 and CXCL10), making this a promising target for improving their outcomes.

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Concepts Keywords
Cancer Biomarkers
Ctla4 Biomarkers, Tumor
Immunotherapy Biomarkers, Tumor
Improved Cancer-immunity cycle
Therapy Chemokine CXCL10
Chemokine CXCL10
Chemokine CXCL9
Chemokine CXCL9
CXCL9 protein, human
Female
Humans
Immune Checkpoint Inhibitors
Immune Checkpoint Inhibitors
Immune checkpoint inhibitors
Immunotherapy
Male
Melanoma
Melanoma
Neoadjuvant Therapy
RNA sequencing
WES

Semantics

Type Source Name
disease MESH melanoma
pathway KEGG Melanoma
disease MESH cancer

Original Article

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