Publication date: May 29, 2025
The wide host range, potential lethality, and zoonotic potential of SARS-CoV-2 infection in animals highlights the need for additional surveillance strategies. We validated a commercial, pH-based, colorimetric RT-LAMP assay for the detection of SARS-CoV-2 RNA in animal feces. The comparator assay was rRT-PCR. The limit of detection of the RT-LAMP assay was 72 genome copies per reaction. RT-LAMP was highly specific for SARS-CoV-2 and did not detect other human or animal coronaviruses. RT-LAMP was robust, with valid results generated for incubation lengths of 30 to 45 min, incubation temperatures of 60 to 70 ^0C, and reaction volumes of 10 to 25 uL. The diagnostic sensitivity was 100% for clinical fecal samples with high viral loads (Ct ≤ 25), 97. 4% for samples with moderate to high viral loads (Ct ≤ 33), and 62% overall (Ct ≤ 40). The diagnostic specificity was 97. 9%. Blinded method testing organized by an independent laboratory confirmed the satisfactory reproducibility of the assay. To our knowledge, this study represents the first validation of RT-LAMP for SARS-CoV-2 detection in animals. RT-LAMP testing could detect SARS-CoV-2 infection more rapidly and at the point of care in animals with moderate to high viral loads, allowing for earlier implementation of control measures.
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Semantics
| Type | Source | Name |
|---|---|---|
| disease | IDO | assay |
| pathway | REACTOME | SARS-CoV-2 Infection |
| disease | MESH | SARS-CoV-2 infection |
| disease | IDO | host |
| disease | MESH | pneumonia |
| disease | MESH | infection |
| disease | MESH | subclinical infection |
| disease | MESH | death |
| disease | MESH | interstitial pneumonia |
| disease | MESH | acute respiratory distress syndrome |
| disease | MESH | fungal infections |
| drug | DRUGBANK | Stavudine |
| disease | MESH | zoonotic spillover |
| drug | DRUGBANK | Gold |
| disease | IDO | replication |
| disease | IDO | nucleic acid |
| drug | DRUGBANK | Phosphate ion |
| disease | IDO | pathogen |
| drug | DRUGBANK | Water |
| drug | DRUGBANK | Cefoxitin |
| disease | MESH | gastroenteritis |
| drug | DRUGBANK | Sumatriptan |
| disease | MESH | viral load |
| drug | DRUGBANK | Lauric Acid |
| drug | DRUGBANK | Tretamine |
| disease | MESH | clinical relevance |